功能性微生物菌剂对花生根际微生物种群的影响

Effects of Functional Microbial Inoculum on Peanut Rhizosphere Microbial Community

为提高花生的产量和品质,对花生障碍地块进行微生物肥料施肥处理,对试验地块施肥前与施肥后进行取样,将样品通过Illumina MiSeq高通量测序,对去重复序列ASVs/OTU进行分析。结果表明:第一次取样样品A(施肥前)与第二次取样样品B(施肥后)属一级相对丰度,在施肥后下降。其中Candidatus solibacter、Mucilaginibacter、Holophaga等几个属相对丰度下降,而Conexibacter、Brevundimonas和Thermus相对丰度表现为增加;多样性指数分析表明,施肥前与施肥后根际微生物多样性存在显著差异,施肥后多样性指数下降;在属水平上,施肥前Mucilaginibacter、Haliangium占比较多,施肥后急剧减少,而Streptomyces、Brevundimonas则显著增加。在两组处理共有的属中Candidatus、Burkholderia、Bacillus和Granulicella占多数。综上所述,微生物肥料的施用对花生土壤有显著的影响,但微生物多样性施肥后有显著下降。

In order to improve the yield and quality of peanut,the field trial was conducted at the peanut soil with biofertilizer.Samples acquired before and after planting were sequenced by high-throughput sequencing Illumina MiSeq method.All the ASVs/OTU were analyzed.The results indicated that,compared with sample A,the relative abundance of sample B was decreasing,especially for the Candidatus solibacter and Mucilaginibacter,Holophaga.But Conexibacter,Brevundimonas and Thermus was increasing.The diversity index analysis showed there was a significant difference between the rhizosphere microbial diversity before and after fertilizing,and the diversity index decreased after fertilizing.At the genus level,the proportion of Mucilaginibacter and Haliangium were getting higher before fertilizing,but decreased sharply after fertilizing,while the relative abundance of Streptomyces and Brevundimonas increased significantly.Candidatus,Burkholderia,Bacillus and Granulicella were the most common genera in the two groups A and B.So the application of microbial fertilizer had a significant impact on microbial community of peanut soil,but the microbial diversity decreased significantly after fertilizing with the microbial fertilizer.