中性粒细胞胞外诱捕网检测技术的研究进展

Overview of detection techniques for neutrophil extracellular traps

中性粒细胞胞外诱捕网(NETs)是近些年新发现的,与自身免疫性疾病、血栓和炎症反应形成有关的网状结构,由中性粒细胞在触发因子诱导下释放产生。不同的刺激物会诱导出不同形式,其数量可被多种检测技术量化。根据检测对象的不同,其分析方法有形态学观察,检测游离DNA和中性粒细胞弹性蛋白酶、髓过氧化物酶等蛋白质组分。本文通过概述这些检测技术及各自优缺点,为进一步拓展NETs在相关疾病的早期诊断、预后判断等方面的应用提供有益参考。

Neutrophil extracellular traps(NETs)are a newly recognized structure in recent years,which is involved in the pathogenesis of autoimmune diseases,thrombosis and inflammatory reaction.NETs can be induced to different forms of reticular structure by a variety of trigger factors and can be quantified by various techniques.According to the different subjects,the analysis methods include morphological observation,detection of cell free DNA and neutrophil elastase,myeloperoxidase,citrullinated histone and other protein components.In this paper,the advantages and disadvantages of these detection techniques are summarized,which will provide useful reference for the further development of the application of NETs in the early diagnosis and prognosis judgment of related diseases.