摘要
目的了解中国脑膜炎奈瑟菌(Neisseria meningitidis,Nm)高致病性ST-4821克隆群(CC4821)荚膜基因簇分布特征,为流行性脑脊髓膜炎的传播扩散机制研究提供依据。方法使用Artemis、MEGA 6、SimPlot和RDP等软件分析41株Nm全基因组序列,分析荚膜基因簇序列差异性、序列重组和基因排布等特征。结果荚膜基因簇ACE区差异较大,重组现象多集中在AC区。D和D′区有明显差异序列,表明这2个区并非精准复制。荚膜基因簇存在基因反转现象。结论Nm高致病性CC4821荚膜基因簇呈现多态性、重组和基因反转等多种荚膜调节机制,可能有助于该克隆群菌株的传播扩散。
Objective To understand the distribution of capsule locus of Neisseria meningitidis clonal complex 4821 in China and provide evidence for the research of spread of meningococcal disease.Methods Software Artemis,MEGA 6,SimPlot and RDP were used to analyze the whole genome sequence of 41 N.meningitidis strains,the sequence divergence of the capsule locus,sequence recombination and genomic rearrangement were studied.Results The ACE regions are highly divergent and likely involved in recombination events.The analysis of the nucleotide sequence of region D and D′showed significant divergence.Finally,this study identified a genomic rearrangement in cps.Conclusion Altogether,this analysis showed that the capsular locus was evolutionary unstable which might be due to recombination events that could lead to capsule switching and have consequences in terms of pathogenesis.
作者
赵盼
朱兵清
张艾煜
徐丽
高源
余建兴
邵祝军
Zhao Pan;Zhu Bingqing;Zhang Aiyu;Xu Li;Gao Yuan;Yu Jianxing;Shao Zhujun(State Key Laboratory of Communicable Disease Prevention and Control,National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《疾病监测》
CAS
2020年第6期508-512,共5页
Disease Surveillance
基金
国家科技重大专项(No.2018ZX10101002–001)
国家自然科学基金(No.81602903)。
关键词
脑膜炎奈瑟菌
CC4821
荚膜基因簇
分布特征
Neisseria meningitidis
Clonal complex 4821
Capsular locus
Distribution characteristic
