ERG、P504s在前列腺穿刺活检标本中的表达及意义

Expression and significance of ERG and P504s in prostate biopsy specimens

目的探讨Ets相关基因ERG蛋白、α-甲酰基辅酶A消旋酶(AMACR,P504s)在前列腺穿刺活检标本中的表达及临床病理意义。方法收集2015年1月至2019年7月安徽省马鞍山市中心医院前列腺穿刺活检标本60例,其中50例确诊为前列腺癌,定义为观察组;另外10例确诊为正常前列腺组织,定义为对照组。采用免疫组织化学法检测活检组织中ERG和P504s蛋白的表达情况,分析其与前列腺癌临床病理特征的关系。结果观察组ERG蛋白阳性表达率稍高于对照组,但差异无统计学意义(24.0%vs 0,χ^2=1.688,P=0.194);P504s蛋白阳性表达率显著高于对照组(96.0%vs 0,χ^2=46.188,P=0.000)。与前列腺癌临床病理特征的关联性分析结果显示,ERG表达与年龄、术前血清前列腺特异抗原(PSA)值无关(P均>0.05),与Gleason分级、国际泌尿病理协会(ISUP)分组及阳性穿刺针数显著相关(P<0.01,P<0.05),且Gleason 3级中的ERG阳性表达率高于Gleason 4级(64.3%vs 8.3%,χ^2=14.370,P=0.000);P504s表达与年龄、Gleason分级、血清PSA值、ISUP分组、阳性穿刺针数均无明显相关性(P均>0.05)。结论ERG与P504s在正常前列腺组织未见表达,在前列腺癌中分别表达和高表达;且ERG表达多在前列腺癌Gleason 3级;ERG和P504s在前列腺癌的诊断中具有一定价值。

Objective To investigate the expressions of ERG(Ets-related gene)-associated protein and alpha-methylacyl-CoA racemase(AMACR,P504 s)in prostate biopsy specimens and their clinicopathological significance.Methods Out of 60 prostate biopsy specimens collected from Maanshan Central Hospital of Anhui Province,there were 50 cases confirmed as prostate cancer(observation group)and 10 cases diagnosed as normal prostate tissue(control group).The immunohistochemistry method was used to detect the expressions of ERG and P504 s in biopsy tissues,and the relationship between the expression levels of ERG and P504 s and the clinicopathological characteristics was analyzed.Results There was no statistical difference in positive expression rate of ERG protein between two groups,even though it was slightly higher in observation group(24.0%vs 0,χ^2=1.688,P=0.194),but the positive expression rate of P504 s protein in observation group was significantly higher than that in control group(96.0%vs 0,χ^2=46.188,P=0.000).The correlation analysis showed that ERG expression was not correlated with age,preoperative serum prostate-specific antigen(PSA)value(all P>0.05),but was significantly correlated with Gleason grading,International Society of Urological Pathology(ISUP)grouping and number of positive punctures(P<0.01,P<0.05),and the positive expression rate of ERG in Gleason grade 3 prostate cancer was higher than that in Gleason grade 4(64.3%vs 8.3%,χ^2=14.370,P=0.000).P504 s expression was not significantly associated with age,Gleason classification,serum PSA value,ISUP grouping,and number of positive punctures(all P>0.05).Conclusions ERG and P504 s are not expressed in normal prostate tissue,but expressed and over expressed in prostate cancer,respectively.ERG expression is mostly in Gleason 3 grade of prostate cancer.Both indexes have certain value in the diagnosis of prostate cancer.