益生菌对卵巢摘除致雌激素缺乏小鼠牙周组织中IL-17表达的影响及其意义

Effect of probiotics on expression of IL-17 in periodontal tissue of estrogen-deficient mice induced by ovariectomy and its significance

目的:探讨益生菌干预对雌激素缺乏小鼠牙周组织中白细胞介素17(IL-17)表达的影响,阐明雌激素缺乏与牙周炎关联性的可能机制。方法:选取8周龄健康雌性C57BL/6J小鼠15只,随机分为假手术组、卵巢摘除组和益生菌干预组(卵巢摘除术后行益生菌灌服),每组5只。采用平均骨密度测量技术检测实验前后小鼠骨矿物质密度(BMD),实时荧光定量PCR(RT-qPCR)法检测小鼠骨髓细胞和牙龈组织中IL-17 mRNA表达水平,流式细胞术检测小鼠骨髓中辅助性T细胞17(Th17)占CD4+TCRβ+细胞的百分率。结果:与假手术组比较,卵巢摘除组小鼠BMD明显降低(P<0.05),骨髓细胞和牙龈组织中IL-17 mRNA表达水平均明显升高(P<0.05),Th17细胞占骨髓CD4+TCRβ+细胞百分率明显升高(P<0.05);与卵巢摘除组比较,益生菌干预组小鼠BMD明显增加(P<0.05),骨髓细胞和牙龈组织中IL-17 mRNA表达水平均明显降低(P<0.05),Th17细胞占骨髓CD4+TCRβ+细胞百分率明显降低(P<0.05);与假手术组比较,益生菌干预组小鼠股骨BMD、骨髓细胞和牙龈组织中IL-17 mRNA表达水平及Th17细胞占骨髓CD4+TCRβ+细胞百分率差异均无统计学意义(P>0.05)。结论:益生菌干预能阻止雌激素缺乏导致的小鼠牙周组织中IL-17水平升高,对雌激素缺乏引起的牙周炎具有预防作用。

Objective:To explore the effect of probiotic feeding on the interleukin-17(IL-17)expression in periodontal tissue of the estrogen-deficient mice,and to elucidate the possible mechanism of the correlation between estrogen deficiency and the periodontitis susceptibility.Methods:A total of 15 female C57BL/J6 mice(8-week-old)were randomly divided into sham operation group,ovariectomy group and probiotics group(probiotics feeding after ovariectomy)(n=5).The bone mineral densities(BMD)of the mice in various groups before and after experiment were measured by mean bone density measurement technique and the IL-17 mRNA expression levels in bone marrow cells and gingiva tissus of the mice were determined by RT-qPCR.The percentages of Th17 cells in CD4+TCRβ+B cells in bone marrow of the mice were measured by flow cytometry.Results:Compared with sham operation group,the BMD of femur of the mice in OVX group was significantly decreased(P<0.01),the expression levels of IL-17 mRNA in bone marrow ce lls and gingiva tissue were increased(P<0.01),and the percentage of CD4+TCRβ+Th17 cells in the bone marrow was increased(P<0.05).Compared with OVX group,the BMD of femur of the mice in probiotics group was significantly increased(P<0.01),the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue were decreased(P<0.01),and the percentage of CD4+TCRβ+Th17 cells in the bone marrow was decreased(P<0.05).Compared with sham operation group,the BMD of femur,the expression levels of IL-17 mRNA in bone marrow cells and gingiva tissue,and the percentage of CD4+TCRβ+Th17 cells in the bone marrow in probiotics group had no significant differences(P>0.05).Conclusion:Probiotics feeding can inhibit the upregulation of IL-17 mRNA level of the mice induced by estrogen deficiency,and has the preventive effect on the estrogen deficiency-induced periodontitis.