HPLC-CAD测定大叶三七叶中人参皂苷Rb1的含量

Determination of ginsenoside Rb1 in the leaf of Daye Sanqi by HPLC-CAD

目的:应用高效液相色谱-电雾式检测器(HPLC-CAD)测定大叶三七叶中人参皂苷Rb1的含量,同时与紫外检测器灵敏度进行比较。方法:采用ThermoHypersil GOLD C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(67∶33),等度洗脱,流速1 mL·min-1,柱温30℃,进样量10 μL。CAD检测器雾化温度Low,数据采集频率10 Hz,过滤常数5.0 s。结果:人参皂苷Rb1在0.070 0~1.409 0μg范围内与峰面积呈良好线性关系(r=0.999 7),平均回收率为100.61%,RSD为1.79%,测得大叶三七叶中人参皂苷Rb1的含量范围为9.2202~13.6232 mg·g-1。结论:本实验所建立的方法灵敏,重复性好,可用于大叶三七叶中人参皂苷Rb1的含量测定。

Objective:To develop a method for determination of ginsenoside Rb1 in the leaf of Daye Sanqi by high performance liquid chromatography coupled with charged aerosol detection (CAD),and to compare the sensitivity of ultraviolet detector(UV) and CAD.Method:Chromatographic separation was performed on a ThermoHypersil GOLD C18 column(4.6 mm×250 mm,5 μm) with methanol-water (67:33).The flow rate was 1 mL·min-1.The column temperature was 30℃,and the injection volume was 10 μL.The detection parameters of CAD detector were as follows:atomization temperature was low,data acquisition frequency was 10 Hz,filter constant was 5.0 s.Result:Ginsenoside Rb1 showed good linear range within 0.0700~1.4090 μg (r=0.9997).The average recovery was 100.61% with RSD of 1.79%.The content of ginsenoside Rb1 was 9.2202-13.6232 mg·g-1.Conclusion:The method is sensitive and reproducible,and can be used for the determination of ginsenoside Rb1 in the leaf of Daye Sanqi.