新生儿缺氧缺血性脑病血清IL-34水平及其与Th17相关细胞因子和NABA评分的相关性

Serum IL-34 level in neonates with hypoxic-ischemic encephalopathy and its correlation with Th17-related cytokines and NABA scores

目的研究新生儿缺氧缺血性脑病(HIE)血清IL-34水平,探讨其在HIE中的临床意义。方法选择宁波市妇女儿童医院2017年1月—2019年12月HIE患儿80例作为HIE组,健康新生儿80例作为对照组。采用双抗夹心酶联免疫吸附试验测定血清IL-34、IL-17、IL-23水平,采用NABA评分评估脑损伤严重程度。结果 HIE组血清IL-34[(24.36±2.41)ng/L]、IL-17[(118.79±9.76)ng/L]、IL-23[(63.24±3.46)ng/L]水平高于对照组[(13.25±2.02)ng/L、(47.53±8.02)ng/L、(15.42±3.12)ng/L],均P<0.05,NABA评分[(30.25±2.02)分]低于对照组[(37.86±1.74)分],P<0.05。不同严重程度HIE患儿血清IL-34、IL-17、IL-23水平和NABA评分比较差异均有统计学意义(均P<0.05),随严重程度增加血清IL-34、IL-17、IL-23水平升高,NABA评分降低。不同NABA评分患儿血清IL-34、IL-17、IL-23水平比较差异均有统计学意义(均P<0.05),随NABA评分的升高,血清IL-34、IL-17、IL-23水平降低。HIE患儿血清IL-34水平与IL-17、IL-23水平呈正相关(均P<0.05)。HIE患儿NABA评分与血清IL-34、IL-17、IL-23水平呈负相关(均P<0.05)。结论 HIE新生儿血清IL-34水平升高,IL-34可能通过与Th17相关细胞因子相互作用,参与患儿脑损伤过程。

Objective To study the serum IL-34 level of neonatal hypoxic-ischemic encephalopathy(HIE)and explore its clinical significance in HIE.Methods Eighty children with HIE were selected as the HIE group and 80 healthy newborns were selected as the control group from January 2017 to December 2019 in Ningbo Women’s and Children’s Hospital.The serum IL-34,IL-17 and IL-23 levels were measured using double-antibody sandwich enzyme-linked immunosorbent assay.NABA scores were used to assess the severity of brain damage.Results The serum levels of IL-34[(24.36±2.41)ng/L],IL-17[(118.79±9.76)ng/L]and IL-23[(63.24±3.46)ng/L]in the HIE group were higher than those in the control group[(13.25±2.02)ng/L,(47.53±8.02)ng/L,(15.42±3.12)ng/L],all P<0.05,and the NABA score[(30.25±2.02)scores]was lower than that in the control group[(37.86±1.74)scores],P<0.05.There were significant differences in serum levels of IL-34,IL-17,IL-23 and NABA scores in children with different severity of HIE(all P<0.05):with the increase of severity of HIE,serum IL-34,IL-17 and IL-23 levels increased,and NABA score decreased.There were significant differences in serum IL-34,IL-17 and IL-23 levels in children with different NABA scores(all P<0.05):with the abnormal degree of NABA score increasing,serum IL-34,IL-17,IL-23 levels decreased.The level of serum IL-34 in children with HIE was positively correlated with the levels of IL-17 and IL-23(all P<0.05).NIE scores in children with HIE were negatively correlated with serum IL-34,IL-17 and IL-23 levels(all P<0.05).Conclusion The serum IL-34 level of HIE neonates is increased,and IL-34 may participate in the brain damage process of children through interaction with Th17-related cytokines.