玄参干预脾虚水湿不化大鼠的脾脏代谢组学研究

Effect of Scrophulariae Radix on Metabonomics of Spleen in Rats with Spleen Deficiency and Water Dampness

目的采用代谢组学方法分析玄参对脾虚水湿不化大鼠脾脏代谢产物的影响,探讨其相关作用机制。方法将SD大鼠按体质量随机分为空白组、模型组及玄参组,每组10只。采用高脂饲料饮食诱导加负重游泳的方法复制脾虚水湿不化大鼠模型,连续6周。玄参组灌胃给予2.9 g·kg-1玄参提取物,其余2组给予等体积生理盐水,每日1次,连续2周。于末次给药12 h后取大鼠脾脏进行组学样本处理,应用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q/TOF-MS)技术寻找潜在的关键性生物标志物,并分析其相关代谢通路。结果玄参可改善脾虚水湿不化大鼠的脾脏代谢产物,共鉴定出与脾虚水湿不化模型及玄参干预后具有共同差异的生物标志物15个,共涉及19条相关的代谢通路。利用MetPA分析发现,影响最为广泛的5条代谢通路可能是甘油磷脂代谢、嘌呤代谢、鞘脂代谢、嘧啶代谢及花生四烯酸代谢。结论玄参对脾虚水湿不化模型大鼠的脾脏代谢产物具有调节作用,该机制可能与玄参具有的利湿功效有关。

Objective Metabonomics technique was used to analyze the effect of Scrophulariae radix on spleen metabolites in rats with spleen deficiency and water dampness,in order to explore the related mechanism.Methods According to body weight,SD rats were randomly divided into three groups:blank group(n=10),model group(n=10)and Scrophulariae radix group(n=10).The rat model of spleen deficiency and water dampness was induced by high-fat diet and weight-bearing swimming for 6 weeks.The rats in the Scrophulariae radix group were given 2.9 g·kg-1 extract by intragastric administration,and the other two groups were given the same volume of normal saline once a day for 2 weeks.At 12 hours after the last administration,the spleens of rats were taken for histology sample treatment.UPLC-Q/TOF-MS technique was used to find potential key biomarkers and related metabolic pathways.Results Scrophulariae radix could improve the metabolic products of spleen in rats with spleen deficiency and water dampness.15 biomarkers were identified,which were different from the model of spleen deficiency,water dampness of spleen deficiency,and the intervention of Scrophulariae radix.The identified biomarkers involved 19 related metabolic pathways.MetPA analysis showed that,the five most extensive interfered metabolic pathways may be glycerol phospholipid metabolism,purine metabolism,sphingomyelid metabolism,pyrimidine metabolism and arachidonic acid metabolism.Conclusion Scrophulariae radix can regulate the metabolic products of spleen in rats with spleen deficiency and water dampness,and the mechanism may be related to the antidampness effect of Scrophulariae radix.