不同施肥条件下蔬菜叶绿素荧光-光谱特性研究

Chlorophyll Fluorescence-Spectral Characteristics of Vegetables Under Different Fertilizer Treatments

测定不同施肥条件下瓢菜、黄白菜的反射光谱及叶绿素荧光参数,以便进一步分析不同施肥条件下蔬菜生长生理及光谱、叶绿素荧光之间的响应关系。结果表明:(1)可见光波段内,黄白菜光谱反射率随生育期的推进呈增加的趋势,瓢菜光谱反射率随生育期的推进呈先增后减的趋势;在近红外波段,瓢菜、黄白菜光谱反射率随蔬菜生长发育逐渐增加。(2)不同施肥条件下蔬菜光谱反射率不同,特别是在近红外波段表现显著。生长期时,黄白菜在C3和C5水平上反射率较高,瓢菜在C2和C3水平上反射率较高;成熟期时,黄白菜在C5和C6水平上反射率较高;瓢菜在C3和C5水平上光谱反射率较高。(3)瓢菜、黄白菜叶绿素荧光参数Fv/Fm随生育期的推进总体呈增加的趋势,瓢菜Fv/Fm在C6水平上最高,黄白菜Fv/Fm在C2和C6水平上较高。(4)不同施肥条件下,蔬菜光谱特征参数与叶绿素荧光都表现出显著的正负相关关系。叶绿素荧光与光谱特性的这种关系,可为监测蔬菜生长生理和健康状况提供参考。

The reflectance spectra and chlorophyll fluorescence parameters of Brassica campestris L. and Brassica pekinensis Rupr. under different fertilization conditions were measured in order to further analyze the growth physiology of vegetables under different fertilization conditions and the response relationship between the spectra and chlorophyll fluorescence. The results show that:(1) In the visible light range, the reflectance spectrum of Brassica pekinensis Rupr. increased with the growth and development of vegetables. The reflection spectrum of the Brassica campestris L. increased first and then decreased with the growth of vegetables;in the near-infrared range, the reflection of Brassica campestris L. and yellow cabbage The spectrum increases with the growth and development of vegetables.(2) Under different fertilization conditions, the spectrum of vegetables was significantly different, and it showed significant performance in the near-infrared band. During the growth period, the reflectance of Brassica pekinensis Rupr. was higher at the C3 and C5 levels, and the reflectance of the Brassica campestris L. at the C2 and C3 levels was high. At the maturity stage, the spectral reflectance of the Brassica pekinensis Rupr. had a higher reflectance at the C5 and C6 levels;the Brassica campestris L. was higher at the C3 and C5 levels.(3) The chlorophyll fluorescence parameters Fv/Fm of Brassica campestris L. and Brassica pekinensis Rupr. increased with the development of the growth period. Fv/Fm of Brassica campestris L. was the highest at C6 level, and Fv/Fm of Brassica pekinensis Rupr. was the highest at C2 and C6 level.(4) Under different fertilization conditions, the characteristic spectral parameters and chlorophyll fluorescence showed a significant positive and negative correlation. The relationship between chlorophyll fluorescence and spectral characteristics can provide a reference for monitoring the growth physiology and health status of vegetables.