钙离子跨膜吸收相关基因在内蒙古白绒山羊胃肠道中的差异表达

Differential Expression of Genes Related to Calcium Ion Transmembrane Absorption in Gastrointestinal Tract of Inner Mongolia White Cashmere Goats

本试验旨在研究钙离子(Ca^2+)跨膜吸收途径中的相关基因在内蒙古白绒山羊胃肠道中的表达差异分析。选用9只4月龄内蒙古白绒山羊断奶羔羊,屠宰后取瘤胃、网胃、瓣胃、皱胃、十二指肠、空肠、回肠、盲肠、结肠、直肠组织样品用于RNA提取,利用实时荧光定量PCR检测维生素D依赖性钙结合蛋白(CaBP-D9k)、瞬时性受体电位通道香草酸受体6(TRPV6)和维生素D受体(VDR)的mRNA表达量。结果表明:CaBP-D9k、TRPV6和VDR mRNA均在十二指肠中表达量最高,CaBP-D9k和TRPV6 mRNA与VDR mRNA分别在大肠内、胃内处于较低的表达水平;CaBP-D9k、TRPV6和VDR mRNA表达量随着小肠的延伸而逐渐降低。综合来看,CaBP-D9k、TRPV6和VDR mRNA表达量与胃肠道Ca^2+跨膜吸收能力存在关联性。

The aim of this study was to investigate the expression pattern of genes related to calcium ion(Ca^2+)transmembrane uptake pathway in gastrointestinal tract of Inner Mongolia white cashmere goats.A total number of 9 weaned Inner Mongolia white cashmere goats(about 4 months old)were slaughtered,and the rumen,reticulum,omasum,abomasum,duodenum,jejunum,ileum,cecum,colon and rectum tissue were obtained for RNA isolation.The mRNA expression of vitamin D-dependent 9 ku calcium-binding protein(CaBP-D9k),transient receptor potential vanilla receptor 6(TRPV6)and vitamin D receptor(VDR)were determined by real-time fluorescence quantitative PCR.The results showed that the mRNA expression of CaBP-D9k,TRPV6 and VDR was the highest in duodenum,while the mRNA expression of CaBP-D9k,TRPV6 and VDR was lower in intestine and stomach,respectively.With the extension of the small intestine,the mRNA expression of CaBP,TRPV6 and VDR were gradually decreased.In conclusion,the mRNA expression of CaBP-D9k,TRPV6 and VDR is correlated with the trans-membrane absorption of Ca^2+in gastrointestinal tract.