发酵糙米乙醇提取物改善3T3-L1脂肪细胞糖脂代谢

Ethanolic Extract of Fermented Brown Rice Improves the Metabolism of Glucose and Lipids in 3T3-L1 Adipocytes

探讨发酵糙米乙醇提取物(FBREE)对胰岛素抵抗3T3-L1脂肪细胞糖脂代谢的影响。高浓度葡萄糖加胰岛素刺激诱导3T3-L1脂肪细胞胰岛素抵抗模型。不同浓度FBREE处理细胞24 h后检测培养液中葡萄糖,游离脂肪酸(FFA),甘油,甘油三酯(TG),肿瘤坏死因子(TNF-α)和白介素6(IL-6)水平。实时定量PCR检测细胞氧化物酶体增殖激活受体γ(PPARγ),CAATT增强子结合蛋白(C/EBPα),固醇调节元件结合蛋白(SREBP1c),葡萄糖转运蛋白4(GLUT4),脂肪酸结合蛋白(aP2),蛋白酪氨酸磷酸酶1B(PTP1B),葡萄糖转运蛋白(GLUT-4),激素敏感脂肪酶(HSL)和TNF-α,IL-6,单核细胞趋化蛋白-1(MCP-1)和C反应蛋白(CRP)的m RNA表达。FBREE具有促进细胞葡萄糖利用,降低细胞内TG含量,减少FFA及甘油溢出的能力。与模型组细胞相比,高浓度FBREE(100μg/m L)处理的细胞中TG和FFA水平分别下降52.44%和37.83%。FBREE同时能减少模型细胞TNF-α和IL-6的分泌,高浓度FBREE(100μg/m L)分别抑制TNF-α(21.18%),IL-6(31.39%),MCP-1(40.09%)和CRP(41.73%) mRNA表达。此外,FBREE有效降低细胞中PPARγ,C/EBPα,SREBP1c,PTP1B,a P2和HSL的m RNA表达,上调GLUT-4m RNA表达。结果提示,FBREE能有效促3T3-L1脂肪细胞消耗葡萄糖,分解TG,减少FFA和甘油溢出;调控细胞糖、脂代谢来改善胰岛素抵抗,并降低胰岛素抵抗所致炎症水平的升高。

The effects of fermented brown rice ethanolic extracts(FBREE) on glucose and lipid metabolism in insulin-resistant 3 T3-L1 adipocytes were investigated. The insulin-resistant model cells were established through the induction by high glucose concentration together with high insulin. The model cells were treated with different concentrations of FBREE for 24 h, and the levels of glucose, free fatty acid(FFA), glycerol, triglyceride(TG), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in the culture medium were determined. The m RNA expression levels of peroxisome proliferator-activated receptors γ(PPARγ), CAATT enhancer binding proteins α(C/EBPα), sterol regulatory element binding protein1 c(SREBP1c), adipocyte fatty acid binding protein 2(aP2), protein tyrosine phosphatase-1 B(PTP1 B), glucose transporter 4(GLUT4), hormone-sensitive triglyceride lipase(HSL), TNF-α, IL-6, monocyte chemotactic protein(MCP)-1 and C-reaction protein(CRP) were determined by quantitative real-time PCR(qRT-PCR) assay. FBREE could promote cellular glucose utilization, decrease the cellular TG content and reduce the released FFA and glycerol in model cells. The high dose of FBREE(100 μg/mL) reduced the cellular TG(by 52.44%) and FFA(by 37.83%), compared with those of the model cells. FBREE also reduced the TNF-α and IL-6 secretion in model cells. A high dose of FBREE(100 μg/mL) also reduced the mRNA expression levels of TNF-α(by 21.18%),IL-6(by 31.39%),MCP-1(by 40.09%) and CRP(by 41.73%) compared with those in model cells. In addition, FBREE was able to reduce effectively the mRNA expressions of PPARγ, C/EBPα,SREBP1 c, PTP1 B, aP2 and HSL, while up-regulating the mRNA levels of GLUT4. These results suggested that the FBREE treatment can promote effectively glucose consumption, TG decomposition, and reduction of FFA and glycerol release, as well as regulate the glucose and lipid metabolism to ameliorate insulin resistance and reduce the increase of inflammation caused by insulin resistance.