麻花秦艽醇提物对高原缺氧大鼠心肌组织的保护作用

Protective effect of ethanol extracts from Gentiana straminea Maxim.on high altitude hypoxia-induced heart damage in rat

目的:研究麻花秦艽醇提物对高原缺氧大鼠心肌组织损伤的保护作用及可能机制。方法:将60只SD大鼠随机分为空白组,模型组,阳性组,麻花秦艽高、中、低剂量组,给药15d,第16天除空白组外均置于低压氧舱,设置低压氧舱海拔为7000m,持续低压24h后取出称重,麻醉后腹主动脉取血并取出心脏称重,计算心脏指数,检测血清和心肌组织各生化指标及心肌组织HE染色做病理检测。结果:与空白组比较,模型组大鼠心脏指数显著升高(P<0.01),cTnT含量、LDH活力、Mb的含量、心肌组织中CK的活力显著增加(P<0.05),心肌组织中SOD、CAT的活力显著降低(P<0.05,P<0.01),MDA、NO含量显著增加(P<0.01,P<0.05);心肌组织中LD含量和LDH的活力显著升高(P<0.05,P<0.01),心肌组织中ATP酶和Na^+-K^+-ATP酶、Ca^2+-Mg^2+-ATP酶含量均显著降低(P<0.01)。与模型组比较,麻花秦艽各剂量组大鼠血清中cTnT、Mb、LDH、心肌组织中CK的含量显著降低(P<0.05,P<0.01);血清与心肌组织中SOD、CAT的活力显著升高(P<0.05,P<0.01),MDA、NO显著降低(P<0.05,P<0.01);心肌组织LD含量显著降低(P<0.01),LDH活力显著降低(P<0.05,P<0.01),ATP酶和Na^+-K^+-ATP酶、Ca^2+-Mg^2+-ATP酶含量显著升高(P<0.05,P<0.01)。麻花秦艽醇提物可以显著改善高原缺氧导致的心肌组织的病理性损伤。结论:麻花秦艽醇提物对高原缺氧大鼠心肌损伤具有良好的保护作用。其机制可能与清除氧自由基、提高抗氧化能力及调节心肌能量代谢有关。

Objective:To study the protective effect and possible mechanism of ethanol extract of Gentiana straminea Maxim.on myocardial tissue injury in rats with high altitude hypoxia.Methods:Sixty SD rats were randomly divided into the blank group,the model group,the positive group,the high-dose group,the medium-dose group and the low-dose group of Gentiana straminea Maxim.were administered for 15 days.On the 16th day,the rats were placed in the low-pressure oxygen chamber except the blank group.The altitude of the low-pressure oxygen chamber was 7000m,after 24 hours of continuous hypotension,blood was taken from abdominal aorta and heart was taken out for weighing.Heart index was calculated,biochemical indexes of serum and myocardial tissue and he staining of myocardial tissue were detected for pathological detection.Results:Compared with the blank group,the cardiac index of the model group was significantly increased(P<0.01),the content of serum cTnT and Mb,LDH activity,CK activity in myocardial tissue were significantly increased(P<0.05),and the activity of SOD and CAT in myocardial tissue were significantly decreased(P<0.05,P<0.01),the content of MDA,NO were significantly increased(P<0.01,P<0.05);increased the content of LD and the activity of LDH in myocardial tissue(P<0.05,P<0.01),the contents of ATPase,Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase in myocardial tissue were significantly decreased(P<0.01).Compared with model group,the levels of cTnT,Mb,LDH in the serum and CK activity in myocardial tissue of rats in the Gentiana straminea Maxim.group were significantly decreased(P<0.05,P<0.01).The activities of SOD and CAT in serum and myocardial tissue were significantly increased(P<0.05,P<0.01),MDA,NO decreased significantly(P<0.05,P<0.01);the content of LD in myocardial tissue decreased significantly(P<0.01)and LDH activity decreased significantly(P<0.05,P<0.01),the contents of ATPase and Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase were significantly increased(P<0.05,P<0.01).The ethanol extract of Gentiana straminea Maxim.can significantly improve the pathological damage of myocardial tissue caused by high altitude hypoxia and reduce the increase of heart index caused by low altitude hypoxia.Conclusion:The ethanol extract of Gentiana straminea Maxim.has a good protective effect on myocardial injury in rats with high altitude hypoxia.The mechanism may be related to scavenging oxygen free radicals,improving antioxidant capacity and regulating myocardial energy metabolism.