滇龙血竭分期干预对肺纤维化大鼠白细胞介素-4与干扰素的影响

Effects of Dian sanguis draconis on IL-4 and IFN-γ serum level in pulmonary fibrosis rats by staged intervention

目的:观察滇龙血竭对肺纤维化(PF)大鼠血清白细胞介素-4(IL-4)、干扰素(IFN-γ)水平的动态影响,探讨其干预PF的作用机制。方法:采用博莱霉素法诱发大鼠PF模型。96只SD大鼠随机分为6组:空白对照组,模型空白对照组,滇龙血竭全程、早期及晚期干预组,阳性药对照组。于制模后1d灌胃相应药物,连续用药并在相应时间点取材。以ELISA法检测血清IL-4及IFN-γ水平。结果:滇龙血竭早期及全程干预可降低血清IL-4水平(P<0.01,P<0.05),提高血清IFN-γ水平(P<0.01,P<0.05)。总体疗效以滇龙血竭全程干预组最佳,与阳性药对照组无显著性差异。结论:滇龙血竭早期及全程干预能够有效减缓博莱霉素诱发的肺泡炎及纤维化进程,其机制可能与调节IL-4/IFN-γ失衡有关。

Objective:To observe the effects of Dian sanguis draconis on the dynamic expression of IL-4 and IFN-γ in serum of pulmonary fibrosis(PF)rats.To explore the possible mechanism of Dian sanguis draconis stage intervention in treating PF.Methods:PF rats models were prepared by intratracheal bleomycin infusion.Ninety-six male Sprague-Dawley(SD)rats were randomized divided into 6 groups,control group,model group,Dian sanguis draconis whole intervention group,Dian sanguis draconis early intervention group,Dian sanguis draconis lately intervention group and pirfenidone group.The rats orally administered continuously with the corresponding drugs 1 day after the model were established,and select materials at the appropriate time point.The levels of IL-4 and IFN-γin serum were detected by ELISA.Results:Early and whole intervention of Dian sanguis draconis could decrease serum IL-4 level(P<0.01,P<0.05)and increase serum IFN-γlevel(P<0.01,P<0.05).The total curative effect was best in the whole intervention group of Dian sanguis draconis,which had no significant difference with pirfenidone group.Conclusion:Early and whole intervention of Dian sanguis draconision can effectively reduce bleomycin-induced alveolitis and fibrosis.The mechanism is related to the regulation of the imbalance between IL-4/IFN-γ.