复方蜥蜴散不同微粒组合剂含药大鼠血清对人胃癌细胞SGC-7901的干预作用

Interventional effect of Compound Lizard Powder with different microparticle combination drug-containing rat serum on human gastric cancer cell line SGC-7901

目的:观察复方蜥蜴散不同微粒组合剂含药大鼠血清对人胃癌细胞SGC-7901增殖、凋亡及周期的影响,探讨其抗癌作用。方法:160只SPF级雄性SD大鼠随机分8组:生理盐水组,复方蜥蜴散80、100目组,复方蜥蜴散80目+100目高、中、低剂量组,华蟾素片组,5-Fu组,每组20只。各组分别予相应药物干预1周后,于腹主动脉处取血,离心,制备含药血清,加于人SGC-7901胃癌细胞培养24h后,MTT法测定人胃癌细胞SGC-7901的增殖活性;采用Annexin V-FITC/PI双染法、流式细胞仪检测人胃癌细胞SGC-7901的凋亡周期。结果:与生理盐水组比较,各药物血清干预组细胞增殖显著受到抑制(P<0.05),细胞总凋亡率显著增加(P<0.05),处于G1期细胞的比例显著增多(P<0.05);与5-Fu组、华蟾素片组比较,各中药组OD均值显著减小(P<0.05),细胞总凋亡率显著增加(P<0.05),处于G1期细胞的比例显著增多(P<0.05);与复方蜥蜴散80目组、100目组比较,复方蜥蜴散80目+100目低、中、高剂量组OD均值显著减小(P<0.05),细胞总凋亡率显著增加(P<0.05),处于G1期细胞的比例显著增多(P<0.05);与复方蜥蜴散80目+100目低、中剂量组比较,复方蜥蜴散80目+100目高剂量组OD均值显著减小(P<0.05),细胞总凋亡率显著增加(P<0.05),处于G1期细胞的比例显著增多(P<0.05)。结论:复方蜥蜴散不同微粒组合剂可诱导人胃癌细胞SGC-7901细胞凋亡,阻滞细胞周期于G1期,有效抑制胃癌细胞生长,具有较好的抗癌作用。

Objective:To observe the effects of Compound Lizard Powder with different microparticle combination drugcontaining rat serum on the proliferation,apoptosis and cycle of human gastric cancer cell line SGC-7901,and explore its anticancer effect.Methods:A total of 160 SPF male SD rats were randomly divided into 8 groups:normal saline group,Compound Lizard Powder 80 mesh group,100 mesh group,Compound Lizard Powder 80 mesh+100 mesh mixed high dose group,middle dose group,low dose group,Huachansu Tablets group,5-Fu group,20 in each group.One week after each group was given corresponding drug intervention,blood was taken from the abdominal aorta and centrifuged to prepare drug-containing serum.After being cultured for 24 hours in human SGC-7901 gastric cancer cells,the proliferation activity of human gastric cancer cell SGC-7901 was determined by MTT assay.The apoptosis cycle of human gastric cancer cell line SGC-7901 was detected by Annexin V-FITC/PI double staining and flow cytometry.Results:Compared with the normal saline group,the cell proliferation of each drug serum group were significantly inhibited(P<0.05),the total apoptosis rate of the cells were significantly increased(P<0.05),the proportion of cells in the G1 phase were significantly increased(P<0.05).Compared with the 5-Fu group and the Huachansu Tablets group,the OD mean of each Chinese medicine group were significantly decreased(P<0.05),the total apoptosis rate of the cells were significantly increased(P<0.05),the proportion of cells in the G1 phase were significantly increased(P<0.05).Compared with the Compound Lizard Powder 80 mesh group,100 mesh group,the OD value of Compound Lizard Powder 80 mesh+100 mesh mixed high dose group,middle dose group,low dose group were significantly decreased(P<0.05),the total apoptosis rate of the cells were significantly increased(P<0.05),the proportion of cells in the G1 phase were significantly increased(P<0.05).Compared with the Compound Lizard Powder 80 mesh+100 mesh mixed low dose group,middle dose group,the OD value of Compound Lizard Powder 80 mesh+100 mesh mixed high dose group was significantly decreased(P<0.05),the total apoptosis rate of the cells was significantly increased(P<0.05),the proportion of cells in the G1 phase was significantly increased(P<0.05).Conclusion:Compound Lizard Powder with different microparticle combination can induce apoptosis of human gastric cancer cell line SGC-7901,arrest cell cycle in G1 phase,effectively inhibit the growth of gastric cancer cells,and have a good anticancer effect.