桉树油化学成分分析及α-松油醇的化感作用

Chemical composition analysis of Eucalyptus essential oil and allelopathic effects of α-terpineol

采用水蒸气蒸馏法提取桉树油,通过GC-MS对其成分进行分析,以其中一种化学成分α-松油醇为代表,分别采用培养皿和盆栽试验研究其对反枝苋的化感作用。结果表明:水蒸气蒸馏法提取桉树油的产率为0.04%,经GC-MS分析,在桉树油中共检测到92种化学成分。在培养皿条件下,α-松油醇对反枝苋的发芽和生长均具有明显的抑制作用,用量达到每皿5和7.5μL时,抑制率均达到100%。盆栽条件下,α-松油醇处理过的土壤会显著影响反枝苋的出苗率、地上鲜重和地上干重,浓度达到1.6μL·cm-3时,抑制作用最大,其化感响应指数分别为-0.51、-0.62和-0.44,抑制率为51%、62%和44%。

We extracted Eucalyptus essential oil by steam distillation and analyzed its chemical components by GC-MS.The inhibitory effects ofα-terpineol,a component in Eucalyptus essential oil,were tested in Petri dishes on the germination and growth of Amaranthus retroflexus.The allelopathic effects ofα-terpineol on A.retroflexus were examined with a pot experiment by measuring germination rate and biomass.The yield of essential oil was 0.04%.Ninety-two chemical components were detected from the essential oil.In Petri dish experiment,the germination inhibitory effect under 5μL and 7.5μL ofα-terpineol per dish reached 100%.In pot experiment,germination,aboveground fresh and dry weights of A.retroflexus were significantly affected byα-terpineol.At the concentration of 1.6μL·cm-3,the inhibition effect was strongest,with the allelopathic response index being-0.51 for germination,-0.62 for aboveground fresh weight,and-0.44 for aboveground dry weight,and the inhibition ratio being 51%,62%and 44%,respectively.