鸭肠炎病毒感染对鸭肠道黏膜组织转录组变化分析

Analysis of Transcriptome Changes in Duck Intestinal Mucosa by Duck Enteritis Virus Infection

为了探究鸭肠炎病毒感染对鸭十二指肠黏膜组织相关免疫基因的变化影响,本试验采用鸭肠炎病毒接种35日龄的健康樱桃谷鸭,将其分为3组(2个试验组和1个对照组),在感染后24 h、48 h采集十二指肠,提取总RNA进行浓度、纯度及完整性检测后构建cDNA文库,使用BGISEQ-500平台对2个实验组和对照组黏膜组织测序,筛选差异表达基因,并用GO和KEGG数据库进行分析。结果显示,2个实验组感染鸭肠炎病毒后T24和T48分别筛选出共720个差异表达基因,其中T24表达上调58个,下调163个;T48表达上调77个,表达下调422个。GO功能注释结果显示,感染后差异基因都与代谢、免疫、炎症和调控途径等密切相关。KEGG通路富集分析发现,感染T24的差异表达基因富集到神经活性配体-受体相互作用、补体和凝血级联、氮代谢、cAMP信号通路和IL-17信号转导通路;感染T48的主要差异表达基因富集到神经活性配体-受体相互作用、细胞因子-细胞因子-受体相互作用、趋化因子信号通路、造血细胞谱系和补体和凝血级联。借助BGISEQ-500平台对鸭肠炎病毒感染后十二指肠黏膜组织相关因子的变化来探究其致病机制及其他生物学通路,弥补了非模式生物转录组研究中缺乏基因信息的不足。

To investigate the effects of duck enteritis virus infection on the changes of duck-duodenal mucosa-associated immune genes,this experiment used duck enteritis virus to inoculate 35-day-old healthy cherry valley ducks,divided them into 3 groups(2 test groups and 1 control group).The duodenum was collected at 24 h and48 h after infection,and total RNA was extracted.The cDNA library was constructed after concentration,purity and integrity detection.The mucosal tissues of the two test groups and the control group were sequenced using the BGISEQ-500 platform,and the differentially expressed genes were screened and analyzed by GO and KEGG databases.The results showed that T24 and T48 of the two test groups were infected with T24 and T48,respectively,and a total of 720 differentially expressed genes were screened.Among them,T24 expression was up-regulated by 58 and down-regulated by 163.T48 expression was up-regulated by 77 and expression was down-regulated by 422.GO functional annotation results show that differential genes after infection are closely related to metabolism,immunity,inflammation and regulatory pathways.KEGG pathway enrichment analysis revealed that differentially expressed genes infected with T24 are enriched in neuroactive ligand-receptor interactions,complement and coagulation cascades,nitrogen metabolism,cAMP signaling pathways and IL-17 signal transduction pathways;T48 infection Major differentially expressed genes are enriched into neuroactive ligand-receptor interactions,cytokine-cytokine-receptor interactions,chemokine signaling pathways,hematopoietic lineage,and complement and coagulation cascades.The BGISEQ-500 platform was used to explore the pathogenic mechanism and other biological pathways of duodenal mucosa related factors after duck enteritis virus infection,which made up for the lack of genetic information in non-model organism transcriptome research.