水稻SDG736抑制拟南芥FLC表达调控开花时间

SDG736 Regulating Flowering Timeby Reducing Expression of FLC in Arabidopsis thaliana

130~150个氨基酸组成SET(Su(var)3-9,Enhancer-of-zeste,Trithorax)结构域构成了组蛋白赖氨酸甲基转移酶特异性催化位点。SET结构域蛋白在进化上高度保守,广泛调控植物的生长发育。进化分析结果显示水稻SET结构域家族成员可分为7个不同的亚家族(KMT1,KMT2,KMT3,KMT6,KMT7,S-ET和RMT)。KMT3亚家族可能涉及开花调控或花的发育,其中包含5个拟南芥基因和5个水稻同源基因。拟南芥SDG4通过H3K4/K36甲基化的活性调控花发育,结果表明水稻同源基因SDG736超量表达,可促进拟南芥开花。对拟南芥开花途径相关的基因进行定量分析显示,超量表达的SDG736拟南芥植株中FLC基因表达量降低,而SCO1基因的表达量增加。

The SET(Su(var)3-9,Enhancer-of-zeste,Trithorax)domain,an evolutionarily conserved motif,approximately contains 130~150 amino acidsthat form a knot-like structure and constitute the enzyme catalytic site for lysine methylation which wide range regulate growth and development processes in plant.Phylogenetic analysis showed that rice SET domain proteins could be divided into eight different subfamilies(KMT1,KMT2,KMT3,KMT6A,KMT6B,KMT7,S-ET and RMT).KMT3 includes five Arabidopsis genes and five rice homologous genes which may be involved in flowering or floral development.SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36.The result showed that overexpression of rice SDG4 homologous genes SDG736 led Arabidopsis early flowering.Flowering gene FLC(Flowering Locus C)was inhibition while SOC1(Suppressor over Expression of CO1)was active.