摘要
为了考察针刺对大脑中动脉闭塞大鼠神经功能和细胞凋亡的影响。将60只SD大鼠随机分为3组,假手术组(Sham)、大脑中动脉闭塞模型组(MCAO)和大脑中动脉闭塞模型+针刺组(MCAO+ACU),每组20只。MCAO+ACU组大鼠采用针刺水沟穴、百会穴和大椎穴进行治疗。研究发现,针刺治疗后,MCAO+ACU组的神经损伤评分显著低于MCAO组(1.33 vs 2.62)(p<0.05)。TTC染色显示,MCAO+ACU组的梗死面积比例显著低于MCAO组(21.53%vs 35.44%)(p<0.05)。TUNEL染色显示,MCAO+ACU组的海马神经细胞凋亡数显著低于MCAO组(62.14 vs 87.46)(p<0.05)。Western blotting结果显示,MCAO+ACU组的p-Raf1、p-MEK2和p-ERK1/2蛋白表达水平均显著低于MCAO组(p<0.05)。因此,本研究表明针刺的神经保护作用机制可能与抑制Raf1/MEK2/ERK1/2信号通路的活化有关。
To investigate the effects of acupuncture on neurological function and apoptosis in rats with middle cerebral artery occlusion.Sixty SD rats were randomly divided into three groups:sham operation group(Sham),middle cerebral artery occlusion model group(MCAO)and middle cerebral artery occlusion model+acupuncture group(MCAO+ACU),20 in each group.Rats in the MCAO+ACU group were treated with acupuncture at Shuigou,Baihui and Dazhui.The study found that after acupuncture treatment,the nerve injury score of MCAO+ACU group was significantly lower than that of MCAO group(1.33 vs 2.62)(p<0.05).TTC staining showed that the infarct size of the MCAO+ACU group was significantly lower than that of the MCAO group(21.53%vs 35.44%)(p<0.05).TUNEL staining showed that the number of hippocampal neuronal apoptosis in MCAO+ACU group was significantly lower than that in MCAO group(62.14 vs 87.46)(p<0.05).Western blotting analysis showed that the expression levels of p-Raf1,p-MEK2 and p-ERK1/2 in MCAO+ACU group were significantly lower than those in MCAO group(p<0.05).Therefore,this study showed that the neuroprotective mechanism of acupuncture may be related to inhibition of activation of the Raf1/MEK2/ERK1/2 signaling pathway.
作者
高玉杰
Gao Yujie(Key Laboratory of Hui Ethnic Medicine Modernization Ministry of Education of Ningxia Medical University,College of Traditional Chinese Medicine of Ningxia Medical University,Yinchuan,750004)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第5期2292-2298,共7页
Genomics and Applied Biology
基金
宁夏医科大学回医药重点实验室开放项目(XY201712)
国家自然科学基金地区项目(81660818)共同资助。