甘草查耳酮A对脑胶质瘤细胞SHG-44凋亡的影响

Effect of Licochalcone A on the Apoptosis of Brain Glioma Cells SHG-44

为了探讨甘草查耳酮A对脑胶质瘤细胞SHG-44凋亡的影响以及其机制,本研究通过CCK-8法检测脑胶质瘤细胞SHG-44细胞活力,使用FITC Annexin/PI双染流式细胞仪检测脑胶质瘤细胞SHG-44凋亡率,通过DCFDA细胞ROS检测试剂盒检测脑胶质瘤细胞SHG-44内活性氧(reactive oxygen species,ROS)水平。结果显示,不同浓度(5μmol/L,10μmol/L和20μmol/L)甘草查耳酮A能明显降低脑胶质瘤细胞SHG-44细胞活力(p<0.05,p<0.01),能显著促进脑胶质瘤细胞SHG-44凋亡(p<0.05);细胞内ROS水平在甘草查耳酮A处理组中明显高于正常脑胶质瘤细胞SHG-44组,ROS抑制剂、NAC预处理可明显抑制草查耳酮A降低的细胞活力(p<0.01,p<0.05),且对脑胶质瘤细胞SHG-44凋亡的促进作用(p<0.01,p<0.05)。本研究结果表明甘草查耳酮A能够通过上调ROS水平促进脑胶质瘤细胞SHG-44凋亡。

To explore the effects of Licochalcone A on the apoptosis of brain glioma cells SHG-44 and its mechanism.Cell Counting Kit-8(CCK-8)was used to detect cell viability;Flow cytometry after FITC Annexin/PI staining was used to detect the apoptosis rate of brain glioma cells SHG-44;DCFDA cellular ROS detection kit was used to detect the ROS(Reactive oxygen species)level of glioma cells SHG-44.Treatment of brain glioma cells SHG-44 with Licochalcone A(5μmol/L,10μmol/L and 20μmol/L)obviously decreased the cell viability of brain glioma cells SHG-44(p<0.05,p<0.01)and significantly promotes the apoptosis rate of brain glioma cells SHG-44(p<0.05);After treatment of brain glioma cells SHG-44 with Licochalcone A(5μmol/L,10μmol/L and20μmol/L),the level of celluar ROS was higher than normal brain glioma cells SHG-44;Pretreatment of brain glioma cells SHG-44 with NAC blocked Licochalcone A-decreased cell viability in brain glioma cells SHG-44(p<0.01,p<0.05)and inhibited Licochalcone A-increased cell apoptosis in brain glioma cells SHG-44(p<0.01,p<0.05).The results of this study showed that Licochalcone A can promote the apoptosis of glioma cells SHG-44 through upreglulating ROS level.