蒙古黄芪不同生育期黄酮类成分积累及其根际微生物多样性研究

Accumulation of Flavonoids and Diversity of Rhizosphere Microorganisms in Astragalus membranaceus var.mongholicus(Bge.)Hsiao in Different Growth Stages

该研究比较了内蒙古赤峰市翁牛特旗、锡林郭勒盟正蓝旗、包头市固阳县、山西省大同市浑源县和甘肃省定西市陇西县5个不同种源的蒙古黄芪不同生育期生长情况及黄酮类成分含量积累,并采用Illumina MiSeq高通量测序技术分析不同生育期根际土壤微生物多样性。结果显示:(1)随着生育期推进,固阳、浑源和赤峰种源的黄芪生长较快,根干重在9月份达到峰值,其他生物量指标种源间差异不大。(2)陇西和浑源种源的黄芪根中毛蕊异黄酮葡萄糖苷含量较高,并在9月份达到峰值,而其他成分(芒柄花苷、毛蕊异黄酮和芒柄花素)呈波动变化。(3)蒙古黄芪不同生育期内根际土壤微生物群落差异较大,其中根际细菌变化最为明显,9月份多样性更高,而真菌群落差异相对较小,不同种源间根际微生物群落组成相似。研究表明,蒙古黄芪毛蕊异黄酮葡萄糖苷含量随生育期显著上升,故最佳采收期不宜过早,而微生物群落结构的差异可能是蒙古黄芪获得生长优势和有效成分积累的重要因素之一。

This study compared the growth and flavonoid accumulation of Astragalus membranaceus var.mongholicus from Wengniute Banner,Chifeng City,Zhenglan Banner,Xilin Gol League and Guyang County,Baotou City of Inner Mongolia Autonomous Region,Hunyuan County,Datong City,Shanxi Province,and Luxi County,Dingxi City,Gansu Province,and analyzed the rhizosphere soil microorganisms by Illumina MiSeq high-throughput sequencing in different growth stages.The results showed that:(1)during growth period,the A.membranaceus var.mongholicus in Guyang,Hunyuan and Chifeng grow faster,root dry weight peaked in September,there was little difference between provenances of other biomass indicators.(2)The content of calycosin-7-O-β-glucoside in the roots of Longxi and Hunyuan provenance was higher and peaked in September.Other components(ononin,calycosin and formononetin)were fluctuating.(3)There was a great influence on the microbial community in the rhizosphere soil at different growth stages of A.membranaceus var.mongholicus.The rhizosphere bacteria had the most obvious influence which the diversity was higher in September,and the difference in fungal communities was relatively small.While the rhizosphere microbial community composition was similar among different provenances.This study showed that the content of calycosin-7-O-β-glucoside of A.membranaceus var.mongholicus was significantly increased during the growth period,so the best harvest period should not be too early.And the difference of microbial community structure may be one of the important factors for obtaining growth advantage and accumulation of active constituents of A.membranaceus var.mongholicus.