抑制SCD1对被动吸烟小鼠肺部炎症的影响

Effects of SCD1 inhibition on the pulmonary inflammation levels of mice with cigarette smoke exposure

目的研究硬脂酰辅酶A去饱和酶1(stearoyl-CoA desaturase 1,SCD1)对被动吸烟小鼠肺部炎症水平的影响。方法将C57BL/6小鼠随机分为对照(CON)组、SCD1抑制剂(CAY)组、吸烟(Smoke)组及吸烟加抑制剂(Smoke+CAY)组,每组11~14只,记录各组小鼠体重及肝脏系数。RT-qPCR及Western blot检测CON组与Smoke组内SCD1表达水平。肺组织HE病理染色、肺泡灌洗液(bronchoalveolar lavage fluid,BALF)蛋白浓度测定及细胞计数比较肺部炎症损伤水平。ELISA检测BALF中TNF-α、IL-6、IL-1β水平。电化学发光法检测血浆中IL-4、IL-10、IL-13水平。结果被动吸烟小鼠肺内SCD1水平升高。给予SCD1抑制剂后,小鼠体重减轻、肝脏系数降低。Smoke+CAY组小鼠较其他组肺部炎症改变明显,BALF中蛋白浓度、细胞计数、TNF-α、IL-6、IL-1β水平均明显升高。各组血浆中IL-4、IL-10、IL-13水平变化不明显。结论吸烟后小鼠肺内SCD1水平升高,且抑制SCD1会加重被动吸烟小鼠肺部炎症损伤。

Objective To investigate the effects of stearoyl-Co A desaturase 1(SCD1)on the pulmonary inflammation levels in mice with cigarette smoke exposure.Methods C57 BL/6 mice were randomly divided into control(CON)group,SCD1 inhibitor(CAY)group,cigarette smoke exposure(Smoke)group and cigarette smoke exposure plus SCD1 inhibitor(Smoke+CAY)group.Each group had11-14 mice.The weight and liver to body ratio of each group were recorded.SCD1 m RNA and protein expression levels of CON group and Smoke group were detected by RT-q PCR and Western blot.HE staining of lung tissues,protein concentration and cell counts of bronchoalveolar lavage fluid(BALF)were performed to determine the degree of pulmonary inflammation among all groups.TNF-α,IL-6,IL-1βin BALF were detected by ELISA.IL-4,IL-10,IL-13 in plasma were detected by electrochemiluminescence assays.Results The expression of SCD1 was upregulated in mice with cigarette smoke exposure.Mice given SCD1 inhibitor injection had lower weight and liver to body ratio.Compared with other groups,mice in Smoke+CAY group had much more apparent changes in lung inflammation,with protein concentration,cell counts,TNF-α,IL-6,IL-1βall elevated in BALF.However,IL-4,IL-10,IL-13 levels in plasma of each group were not significantly changed.Conclusion Expression of SCD1 was elevated in lung tissues of mice with cigarette smoke exposure.Inhibition of SCD1 aggravated the lung inflammatory injury in mice with cigarette smoke exposure.