低能量强脉冲光影响C57BL/6小鼠毛发生长的实验研究

Effects of Low Energy Intense Pulse Light on Hair Growth in C57BL/6 Mice

目的:观察低能量强脉冲光(IPL)对C57BL/6小鼠毛发生长及生长周期的影响。方法:制作小鼠休止期脱毛模型,将35只小鼠随机分为阳性对照组、空白对照组、IPL高能量组、IPL中能量组、IPL低能量组,对IPL高能量组、IPL中能量组、IPL低能量组脱毛区进行IPL照射,比较各组小鼠毛发生长情况。实验14、21 d,取各组鼠背脱毛区皮肤行HE染色及毛囊计数,观察IPL照射对毛囊生长的影响。结果:IPL低能量组毛发长度显著长于其他组,每倍视野毛囊数为(68.9±5.4)个,明显多于其他组,差异均有统计学意义(P<0.05)。结论:低能量IPL照射可能是促进毛发生长的一种有效治疗手段。

Objective:To observe the effects of low energy intense pulse light(IPL)on hair growth and growth cycle in C57BL/6 mice.Method:The depilation model of telogen was made,thirty-five mice were randomly divided into the positive control group,the blank control group,the IPL high energy group,the IPL medium energy group and the IPL low energy group.IPL irradiation was performed on the depilation area of the IPL high energy group,the IPL medium energy group and IPL low energy group.Hair growth of mice in each group was compared.On day 14 and 21,HE staining and hair follicles counting were performed on the depilated area of each group,and the effect of IPL irradiation on hair follicles growth was observed.Result:The length of the hair in IPL low energy group was significantly longer than those of the other groups,and the number of follicles per visual field was(68.9±5.4),which was significantly more than those of the other groups,and the differences were statistically significant(P<0.05).Conclusion:Low intensity IPL irradiation may be an effective treatment for hair growth.