摘要
Syntaxin-1是一种多结构域蛋白,通过与synaptobrevin-2和SNAP-25形成SNARE复合体调节囊泡融合.然而,syntaxin-1在突触形成过程中是否发挥作用,目前尚不清楚.本研究显示syntaxin-1的表达水平与突触形成过程高度相关.Syntaxin-1的R151A和I155A突变影响其在突触形成中的促进作用,而Habc结构域或跨膜结构域在突触形成中无显著作用.结果表明,syntaxin-1通过激活突触囊泡释放来加速突触的形成.
yntaxin-1, known as a multi-domain protein, regulates vesicle fusion by forming SNARE complex with synaptobrevin-2 and SNAP-25. However, the role of syntaxin-1 in synapse formation remains uncovered.Here we demonstrated that the expression level of syntaxin-1 was highly associated with the process of synaptogenesis. The R151A and I155A mutations but not Habcor transmembrane domain truncations of syntaxin-1 impaired its facilitation in synapse formation. Our results suggested that syntaxin-1 accelerated synapse formation via activating synaptic vesicle release.
作者
裴青
林寅
荣伊
刘孟雪
吴世平
阳小飞
PEI Qing;LIN Yin;RONG Yi;LIU Meng-Xue;WU Shi-Ping;YANG Xiao-Fei(Key Laboratory of Cognitive Science,Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis&Treatment,Laboratory of Membrane Ion Channels and Medicine,College of Biomedical Engineering,South-Central University for Nationalities,Wuhan 430074,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2020年第7期626-633,共8页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金(31670850)
中南民族大学基础研究基金(CZY19022和CZY19040)资助项目~~。
