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异莲心碱对HepG2细胞周期和凋亡的影响 被引量:1

Effects of Isoliensinine on the Cycle and Apoptosis of HepG2 Cells
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摘要 目的:研究异莲心碱对人肝癌Hep G2细胞周期与凋亡的影响。方法:采取流式细胞术检测不同浓度异莲心碱诱导Hep G2细胞24 h后对周期的影响;Hoechst33258染色观察异莲心碱诱导Hep G2细胞凋亡后细胞核形态的变化;JC-1染色检测细胞线粒体膜电位变化;蛋白免疫印记法(Western blot)检测Bax、Bcl-2蛋白的表达量。结果:流式结果显示,G0/G1期和G2/M期比例明显升高,而S期比例明显下降,并呈现剂量依赖性;Hoechst33258结果表明,药物诱导Hep G2细胞后,细胞数量减少,形态皱缩或改变,蓝色荧光加深;Western bolt检测可知,异莲心碱上调Bax蛋白并下调Bcl-2蛋白的表达水平。结论:异莲心碱可使Hep G2细胞阻滞于G0/G1期、G2/M期,诱导Hep G2细胞发生早期凋亡,其作用可能与Bax、Bcl-2两种凋亡蛋白表达水平变化相关。 Objective:To study the effects of isoliensinine on the cycle and apoptosis of Hep G2 cells.Methods:Flow cytometry was applied to determine the influence of isoliensinine at different concentrations on the cycle of Hep G2 cells after 24 hours.Hoechst33258 staining was utilized to observe the changes of nuclear morphology of Hep G2 cells induced by isoliensinine.The mitochondrial membrane potential changes of Hep G2 cells was detected by JC-1 staining.The expressions of Bax and Bcl-2 protein were observed by Western blot.Results:Flow cytometry indicated that the ratios of G0/G1 and G2/M phases of the cell cycle increased,while the ratio of S phase decreased induced by isoliensinine in a dose-dependent manner.Hoechst33258 results showed that the number of Hep G2 cells decreased and the shape shrank or changed,and the blue fluorescence intensity increased.Western bolt showed that the expressions of Bax proteins were up-regulated,while those of Bcl-2 proteins were down-regulated.Conclusion:Isoliensinine arrests in G0/G1 and G2/M phases and induces the early apoptosis of Hep G2 cells,which may be related to the changes in the expressions of Bax and Bcl-2 proteins caused by isoliensinine.
作者 侯亚婷 李天一 宋金春 Hou Yating;Li Tianyi;Song Jinchun(Renmin Hospital of Wuhan University,Wuhan 430060,China;School of Pharmaceutics,Shihezi University)
出处 《中国药师》 CAS 2020年第7期1261-1264,共4页 China Pharmacist
关键词 异莲心碱 Hep G2细胞 周期 凋亡 Isoliensinine Hep G2 cells Cycle Apoptosis
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