介孔生物玻璃微球/羧甲基纤维素复合可吸收止血材料

Nanocomposites Composed of Mesoporous Bioglass Nanoparticles and Carboxymethyl Cellulose for Hemorrhage Control

以羧甲基纤维素(CMC)为主要成分的纤维素基可吸收止血材料已获得广泛应用,为进一步提高CMC的止血性能,采用冷冻干燥法制备了介孔生物玻璃微球(mBN)/CMC纳米复合海绵,并对其形貌、结构及性能进行了表征。结果显示,mBN为平均粒径100 nm左右、粒径分布均一的规则球体,内部有介孔结构,比表面积达852 m2/g;mBN的引入增加了复合材料的孔径、孔隙率和表面负电荷密度。动态全血及血浆凝固实验表明,mBN的引入主要通过内源性途径显著提高了材料的体外促凝血效果,而对外源性和共通途径无显著影响。细胞实验结果表明,与CMC相比,mBN/CMC细胞相容性更好,并对细胞的增殖有促进作用。mBN/CMC有望作为高性能可吸收止血材料获得临床应用。

Cellulose-based absorbable hemostatic materials with the main component of carboxymethyl cellulose(CMC)have been widely developed.In order to improve the hemostatic performance of CMC,mesoporous bioglass nanoparticles(mBN)/CMC nanocomposite sponge was fabricated via lyophilization,and its morphology,structure and performance were characterized.The results reveal that mBN is spherical nanoparticles with an average diameter about 100 nm,extremely uniform particle size distribution and mesoporous structure inside,and its specific surface area is 852 m2/g.The introduction of mBN increases the pore size,porosity and negative surface charge density of the sponges.The whole blood clotting and plasma coagulation experiments indicate that mBN accelerates in vitro coagulation via the intrinsic pathway,does not significantly affect the extrinsic and common pathway.The cell experiment shows that mBN/CMC has better cell compatibility and promotes cell proliferation,compared with CMC.mBN/CMC is an promising absorbable hemostatic material with high performance for clinical application.