2015-2019年许昌市HIV抗体免疫印迹试验不确定检测结果分析

Analysis of the uncertain results of HIV immunoblotting test in Xuchang from 2015 to 2019

目的通过对HIV抗体不确定者的随访检测结果进行分析,从中探寻两种补充试验对HIV抗体疑难样本结果的鉴别效果。方法对2015-2019年许昌市69例HIV抗体为不确定结果的带型进行鉴别分析,经随访抗体检测和病毒载量检测,同时结合其血清学特点及流行病学史。结果复检两种筛查试剂检测结果不一致的有21例,病毒载量基线检测结果均为阴性;在二次随访检测中2例阳转,19例排除HIV感染。复检两种筛查试剂检测结果均为阳性的有48例,35例病毒载量基线检测结果为阳性;在二次随访检测中34例确证为HIV抗体阳性,1例在一年内多次随访检测中WB带型均无进展,结合病毒载量检测结果和流行病学史最终确证为HIV抗体阳性。结论对HIV抗体检测中WB带型结果不确定样本要谨慎对待,应结合随访抗体检测和病毒载量检测结果来鉴别HIV的感染状态,确保检测结果的准确性。

Objective To explore the effect of two supplementary tests on the identification of difficult HIV antibody samples,through the analysis of follow-up test results of people with uncertain HIV antibodies.Methods 69 cases of uncertain HIV antibodies in Xuchang from 2015 to 2019 were identified,followed up by antibody detection and viral load detection,combined with their serological characteristics and epidemiological history.Results There were 21 cases where the results of the two screening reagents were inconsistent,and the baseline viral load test results were negative;2 cases were positive in the second follow-up test,and 19 cases were excluded from HIV infection.Re-examination of the two screening reagents were positive in 48 cases,35 cases of viral load baseline test results were positive;in the second follow-up test,34 cases were confirmed to be HIV positive,and 1 case was confirmed to be HIV positive in multiple follow-up tests within one year.Conclusion The samples with uncertain WB banding results in HIV antibody testing should be treated with caution.Follow-up antibody testing and viral load testing should be used to identify HIV infection status to ensure the accuracy of the test results.