摘要
从马铃薯田自然罹病马铃薯块茎蛾幼虫上,分离到1株对马铃薯块茎蛾幼虫具有较强致病作用的菌株ML-1,通过形态及16S r RNA序列测定分析,确定该菌株ML-1为黏质沙雷氏菌Serratia marcescens。采用饲喂法分别测定了该菌株ML-1菌悬液、发酵液及发酵上清液对马铃薯块茎蛾初孵3日龄幼虫的毒力。结果表明,用ML-1菌悬液、发酵液及发酵上清液饲喂后第7 d时,马铃薯块茎蛾3日龄幼虫的累积校正死亡率分别为63.16%、80.70%和49.12%,在3.0×109 cfu/m L浓度下的LT50分别为4.71、3.04和6.47 d,在发酵液处理后第3、5和7 d的LC50分别为9.784×1010、1.855×108和5.434×105 cfu/m L。综上所述,黏质沙雷氏菌ML-1菌株对马铃薯块茎蛾幼虫有较强的毒力,在马铃薯块茎蛾绿色防控中具有一定的开发潜能。
A strain ML-1 showing strong pathogenicity to larvae of potato tuber moth was isolated from the natural diseased larvae of potato tuber moth collected from potato fields.The strain ML-1 was identified as S.marcescens by morphological observation of pathogens and 16S rRNA sequencing analysis.The virulence of the strain ML-1 suspension,fermentation broth and fermentation supernatant to the 3-d-old larvae of potato tuber moth was determined by feeding method.The cumulative corrected mortalities were 63.16%,80.70%and 49.12%on the 7th day after feeding,respectively,and the LT50 were 3.04,4.71 and 6.47 d at 3.0×109 cfu/mL,respectively.The LC50 at the 3rd,5th and 7th day after treatment with fermentation broth were 9.784×1010,1.855×108 and 5.434×105 cfu/m L,respectively.In summary,S.marcescens ML-1 strain has strong virulence to potato tuber moth larvae and has good prospective for development of biological control agents against the pest.
作者
苏造堂
张凌英
徐天梅
杜广祖
陈斌
肖关丽
SU Zaotang;ZHANG Lingying;XU Tianmei;DU Guangzu;CHEN Bin;XIAO Guanli(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan/College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Agriculture&Biology Technology,Yunnan Agricultural University,Kunming 650201,China)
出处
《中国生物防治学报》
CSCD
北大核心
2020年第3期361-370,共10页
Chinese Journal of Biological Control
基金
国家自然科学基金(31760519,31660537)。
关键词
马铃薯块茎蛾
黏质沙雷氏菌
毒力测定
致病力
杀虫活性
potato tuber moth
Serratia marcescens
virulence determination
pathogenicity
insecticidal activity