摘要
解淀粉芽胞杆菌Bacillus amyloliquefaciens PHODG36是一株具有抑菌和促生作用的多功能菌株。本研究以菌株PHODG36原粉为有效成分、滑石粉为填料研制了不同芽胞浓度的微生物菌剂,通过盆栽试验研究了菌剂拌种处理马铃薯种薯后对其出苗的影响,在冀北和冀南地区开展田间小区试验研究了该菌剂对马铃薯黄萎病的防治效果及对马铃薯产量的影响。结果表明,制备的PHODG36菌剂外观为浅黄色粉末,无团块。该菌剂平均粒径D50为(38.25±0.46)μm,p H为(6.65±0.15),贮藏后未出现结块和发粘现象,浓度介于1.5×109~2.0×1010 CFU/g。与空白对照和化学药剂相比,不同浓度菌剂拌种处理后校正出苗率之间差异不显著,而菌剂随着浓度的增加,校正出苗率呈下降趋势。不同地区的田间试验表明,PHODG36菌剂在1.5×109~1.0×1010 CFU/g对感病品种黄萎病的病情指数防效为43.08%~58.46%,增产率为8.29%~30.37%;对抗病品种主要表现为增产作用,增产率介于1.85%~9.0%。综合分析表明,菌剂使用浓度为1.5×109~1.0×1010 CFU/g能够有效降低黄萎病的发生,节约投入成本,且增产作用明显。研究结果为黄萎病的防控提供绿色产品,并为该菌剂进一步的大面积推广示范应用奠定了基础。
Bacillus amyloliquefaciens PHODG36 is a multifunctional strain which has significant control efficiency on diseases and growth-promotion effect on plants.In this study,microbial agent with different spore concentrations was prepared using strain PHODG36 raw powder as active ingredient and talc powder as filler.Pot experiments were conducted to investigate potato seedling emergence after seed dressing,and field plot experiments were carried out to investigate the effect of microbial agent PHODG36 on control efficacy of potato Verticillium wilt and potato yield in northern and southern Hebei Province.The results showed that microbial agent PHODG36 had a light yellow appearance and no agglomeration.The microbial agent had an average particle size of(38.25±0.46)μm and p H value of(6.65±0.15).There was no caking and sticking after storage for the agent,and the concentrations were ranged from 1.5×109 to 2.0×1010CFU/g.Compared with control and chemical treatment,there was no significant difference on the corrected emergence rate among different concentrations of microbial agent PHODG36 after seed dressing,but the corrected emergence rate decreased with the increase of concentration.Field experiments in different areas showed that the control efficacy on disease index of Verticillium wilt was 43.08%to 58.46%,and the yield increased from 8.87% to 30.37%for susceptible varieties,1.85%~9.0%for resistant varieties,when the concentration of microbial agent PHODG36 were ranged from 1.5×109 to 1.0×1010CFU/g.Comprehensive analysis showed that the application of microbial agent PHODG36 at 1.5×109 to 1.0×1010 CFU/g could effectively reduce the incidence and disease index of potato Verticillium wilt,save the cost of investing,and significantly increase production.The results indicated that green products were provided for control of Verticillium wilt,which laid a foundation for the further extensive application of microbial agent PHODG36.
作者
赵卫松
郭庆港
张晓云
王培培
苏振贺
胡卿
鹿秀云
马平
李社增
ZHAO Weisong;GUO Qinggang;ZHANG Xiaoyun;WANG Peipei;SU Zhenhe;HU Qing;LU Xiuyun;MA Ping;LI Shezeng(IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,China)
出处
《中国生物防治学报》
CSCD
北大核心
2020年第3期381-387,共7页
Chinese Journal of Biological Control
基金
国家公益性行业(农业)科研专项(201503109)
国家重点研发计划(2017YFD0201101)
河北省农林科学院现代农业科技创新工程课题(1-02-03)。
关键词
解淀粉芽胞杆菌
马铃薯黄萎病
出苗
防治效果
增产
Bacillus amyloliquefaciens
potato Verticillium wilt
seedling emergence
control effect
yield increase