长链非编码RNA PTENP1对NSCLC细胞增殖和转移的影响

Effects of Long-chain Non-coding RNA PTENP1 on Proliferation and Metastasis of NSCLC Cells

目的探究长链非编码RNA PTENPI对非小细胞肺癌(NSCIC)细胞增殖和转移的影响。方法收集2017年6月至2018年6月在济宁市第一人民医院确诊的30例NSCLC患者的肿瘤组织及癌旁正常组织,采用实时定量PCR(RT-PCR)技术检测组织表达水平。将非小细胞肺癌BEAS-2B细胞分为Control组(未转染).NC组(转染慢病毒载体)及PTENPI组(转染负载PTENPI基因的慢病毒载体),检测各组细胞PTENPI表达水平,比较各组细胞增殖、克隆、调亡。迁移、侵袭能力及PTEN.p-Akt.Akt蛋白表达水平。10只大鼠随机分为Control组及PTENI组,5只/组,分别皮下注射Control组及PTENI组BEAS-2B細胞,在喂养5.10、15.20.25及30 d测量各组大鼠肿瘤体积,30 d后断颈处死大鼠。取出肿瘤组织并检测其PTENPI表达水平及PTEN.p-Akt阳性细胞占比。结果NSCIC患者肿瘤组织PTENPI表达水平高于癌旁正常组织,PTENPI组细胞PTENPI表达水平高于NC组(P均<0.01)。NC组细胞Aso,细胞克隆数、细胞凋亡率、划痕愈合率、细胞侵袭率与Control组无明显差异(P均>0.05),但均高于PTENPI组(P均<0.01)。NC组细胞PTEN蛋白相对表达量及p-Ak/Akt比值与Cntrol组无明显差异(P均>0.05);PTENI组PTEN蛋白相对表达量高于NC组,p-AkV/Akt比值低于NC组(P均<0.01)。喂养30d后,PTENI大鼠肿瘤体积小于NC组,肿瘤组织PTENPI表达水平及PTEN阳性细胞占比高于NC组,p-Aki阳性细胞占比低于NC组(P.<0.01)。结论长链非编码RNA PTENPI可有效抑制非小细胞肺癌細胞增殖和转移,并诱导凋亡,面其作用机制可能与提升PTEN表达进而激活PI3K/Akt信号通路有关。

Ohjective To investigate the efeee of long-chain non-oding RNA PTENPI on proliferation and metastasis of non-small cell lung eaneer(NSCLC)ells.Methods The tumor tissues and adjacent normal issues of 30 NSCLC patients who were confirmed in the hospital from June 2017 to June 2018 were ollected.The expression level of PTENPI in issues was detected by real-time quantitative PCR(RT-PCR)。NSCLC BEAS-2B cells were divided into Control group(untransfected),NC group(tanslected with lentiviral vector)and PTENPI group(transeeted with lentiviral vector carrying PTENPI gene).'The expression level of PTENPI in each group was detected.The cell proliferation,cloning,apoptosis.migration,invasion ability and expression lev-els of PTEN,p-Akt and Akt were compared among all the groups.Ten rats were randomly div ided into Control group and PTENI group,5 cases in each group.The two groups were subecutaneously injected with BEAS-2B cells from the Control group and PTENI group,respectively.Tumor volume of rats in each group was measured5,10,15,20,25 and 30 days ater feding.Thirty days lat-er,they were sarifeed and the tumor tssues were taken out.Expression level of PTENPI,and proportion of PTEN and p-Akt positive cells were dected.Results The expression level of PTENPI in tumor tssues of NSCLC patients was higher than that in adjacent normal issues.The ex-pression level of PTENPI in PTENPI group was higher than that in NC group(P<0.01).There was no significant difference in OD450,number of cell cloning,apoplosis rate,scratch healing rate or cell invasion rate between NC group and Control group(P>0.05).However,the above in-dexes in the above two groups were higher than those in PTENP1 group(P<0.01).There was no significant difference in relative expression quantity of PTEN protein or p-Akt/Akt between NC group and Control group(P>0.05).The relative expression quantiy of PTEN protein in PTENI group.was higher than that in NC goup,while p-Ak/Akt was lower than that in NC group(P<0.01)After 30 days of feeding,tumor volume of PTENI rats was smaller than that of NC group,while ex-pression level of PTENPI in tumor tsses and proportion of PTEN positive cells were higher than those of NC group,and proportion of p-Akt positive cells was lower than that of NC group(P<0.01).Conclusion Long-chain non-coding RNA PTENPI can efectively inhibit proliferation and metastasis of NSCLC cells,and induce apoplosis.And its mechanism of action may be related to promoting PTEN expression thus activating PI3 K/Akt signaling pathway.