微囊化酿酒酵母FM-S-115的高密度培养

High Cell Density Culture of Saccharomyces cerevisiae FM-S-115 in Microencapsulation

在单因素实验的基础上,采用正交试验对培养基和培养条件优化,并结合微胶囊化培养方法,以实现酿酒酵母FM-S-115的高密度培养。结果表明,单因素实验对碳源和碳源含量、氮源和氮源含量、温度、pH和菌液接种量七个条件的优化确定三个影响最显著的因素为:温度、pH和碳源含量。通过三因素三水平正交试验得出最适培养条件:温度是32℃,pH为4.5,碳源为蔗糖且含量为16%,在此条件下培养计得活菌总数为1.79×10^8CFU/m L,是正常培养条件下1.02×10^8CFU/m L的1.75倍。通过计算不同质量浓度海藻酸钠和氯化钙制得的微胶囊破囊率得出,海藻酸钠质量浓度为1.2%,氯化钙质量浓度为7%时,破囊率最低。将微囊化酵母在最适条件下静置培养四代,菌落总数为8.10×10^8CFU/g,是正常培养的7.94倍,是在最适条件下摇床培养的4.53倍,菌体密度得到大幅提升。

On the basis of single factor experiments,orthogonal experiments were used to optimize the medium and culture conditions,and microencapsulation culture method was combined to achieve high density culture of Saccharomyces cerevisiae FM-S-115.Results showed that,by optimizing seven conditions of carbon source and carbon source content,nitrogen source and nitrogen source content,temperature,pH and inoculum concentration,single factor experiment determined the three most significant factors:Temperature,p H and carbon source content.Through the orthogonal experiment of three factors and three levels,the optimum culture conditions was determined:Temperature was 32℃,p H was 4.5,carbon source was sucrose and the content was 16%.Under this culture condition,the total number of colonies was 1.79×10^8 CFU/m L,which was 1.75 times of the colony number of 1.02×10^8 CFU/m L under normal culture condition.By measuring the capsule breaking rate of microcapsules prepared by sodium alginate and calcium chloride with different mass concentrations,the capsule damage rate was the smallest when the mass concentration of sodium alginate was 1.2%and the mass concentration of calcium chloride was7%.FM-S-115 in microencapsulation cultured four generations under the optimal conditions for static,the total number of colonies was 8.10×10^8 CFU/g,which was 7.94 times that of normal culture and 4.53 times that of culture under the optimal conditions.The cell density was greatly improved.