软水蓼不同目数超微粉体溶出物的化学成分分析

Analysis of Chemical Constituents of the Extractives from Polygonum hydropiper L.with Different Number of Ultra-fine Powder

为分析软水蓼不同目数粉体溶出物的化学成分。采用超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF/MS)对软水蓼不同目数粉体化学成分进行初步分析。结果表明:软水蓼不同目数粉体的体积粒径大小随目数增加而降低;面积平均粒径从50目下降至300目时最低,再逐渐增加;粉体颗粒表面随目数增加越来越光滑,原药材特征越来越不明显;不同目数粉体溶出物中分离鉴定出17个化合物,其中黄酮类化合物9个、苯丙素类5个、有机酸、多糖及鞣质各1个,其中苯丙素类化合物Vanicoside C在200目和300目粉体中没有检测出;黄酮类化合物的峰面积值从过50目筛的粉体逐渐升高,在120目出现较高的峰面积值,随后从200~300目逐渐降低,再到500目时呈逐渐升高趋势,其中槲皮苷的相对峰面积值最高为47.11%;苯丙素类化合物峰面积值从50目下降至300目最低值,随后呈上升趋势,其中Vanicoside B相对峰面积值最高为47.79%,为明确活性化学成分作用机制及药效物质基础的阐明提供了重要依据。

In order to analyze the chemical constituents of the extracts of Polygonum hydropiper L. with different mesh numbers. The chemical constituents of Polygonum hydropiper L. with different numbers were analyzed by time of flight mass spectrometry with super-high-performance liquid chromatography pole(UPLC-Q-TOF/Ms). The volume size of Polygonum hydropiper L. powder with different mesh decreased with the increase of mesh number;the average area size decreased from 50 mesh to 300 mesh,and then increased gradually;the surface of powder particles became more and more smooth with the increase of mesh number,and the characteristics of original medicinal materials became less and less obvious.17 compounds were isolated and identified from different mesh powder extracts,including 9 flavonoids,5 phenylpropanoids,1 organic acid,polysaccharide and tannin respectively,among which Vanicoside C was not detected in 200 mesh and 300 mesh powder;the peak area value of flavonoids gradually increased from the powder passing through the 50 mesh sieve,a higher peak area value appeared at 120 mesh,then gradually decreased from 200 to 300 mesh,and gradually increased to 500 mesh. Among them,the relative peak area value of quercetin was 47.11%;the peak area value of phenylpropanoid compounds dropped from 50 mesh to the lowest value of 300 mesh,and then showed an upward trend,of which Vanicoside B relative peak area value was 47.79%. It provides an important basis for clarifying the action mechanism of active chemical components and the basis of effective substances.