超低温联合亚常温机械灌注保存肝脏实验技术研究进展

Research progress in supercooling and subnormothermic machine perfusion technique in liver preservation

目前临床上应用最广泛的肝脏保存方式为静态低温保存技术,理论上仅可保存肝脏12 h。随着静态低温保存时间的延长,肝脏的活性会逐步下降。这限制了器官的运输和匹配。超低温(不结冰)保存联合亚常温机械灌注技术可以显著延长大鼠肝脏的有效保存时间长达3~4 d。这对保证肝脏的公平分配,降低肝脏的废弃率,具有极大的现实意义和临床价值。本文综述了超低温联合亚常温机械灌注保存肝脏实验技术研究进展。

The most widely used method of liver preservation is static cryopreservation(SCS),which can only preserve livers for 12 h in theory.With the extension of the static cryopreservation time,the activity of livers will gradually decline.This limits the transportation and matching of organs.The storage time of rat livers can be significantly prolonged up to 3-4 days by combining supercooling(subzero nonfreezing)and subnormothermic machine perfusion(SNMP)while using the cryoprotectants.It is of great practical significance and clinical value to ensure the fair allocation of liver and reduce the wastage rate.This review summarizes the progress in supercooling and subnormothermic machine perfusion technique in liver preservation.