白细胞介素6和磷酸化信号转导及转录活化因子3在B族链球菌阳性孕妇中表达

Expression of interleukin-6 and phosphorylated signal transduction and activator of transcription 3 in pregnant women with group B Streptococcus positive

目的探讨B组链球菌(GBS)阳性的早产孕妇中白细胞介素6(IL-6)和磷酸化信号转导及转录活化因子3(p-STAT3)的表达及其与早产的关系。方法选择深圳市福田区妇幼保健院2018年6月至2019年6月门诊产前检查至住院待产的孕妇1950例中GBS阳性者共122例。对GBS阳性早产20例(早产组)、足月胎膜早破者33例(胎膜早破组)、足月阴道分娩孕妇66例(足月分娩组)、流产3例(流产组)及同期GBS阴性孕妇20例(GBS阴性对照组)的阴道分泌物制备悬浮液,采用ELISA法检测IL-6浓度,Western blot检测p-STAT3、p-Akt和NF-κB亚基p65的表达。结果入组孕妇GBS阳性率为6.25%(122/1950),其中流产、早产、足月胎膜早破和足月分娩孕妇分别占2.45%(3/122)、16.12%(20/122)、26.62%(33/122)和53.66%(66/122)。GBS阳性早产组孕妇IL-6表达[(0.065±0.034)pg/ml]显著高于GBS阳性足月产者[(0.045±0.021)pg/ml](t=-3.192、P=0.002)和GBS阴性者[(0.043±0.020)pg/ml](t=-2.494、P=0.017),差异均有统计学意义。p-STAT3在GBS阳性早产病例中的表达(灰度值:0.06±0.03)显著高于GBS阴性对照组(灰度值:0.04±0.01),差异有统计学意义(t=-2.981、P=0.005);GBS阳性早产组和GBS阴性对照组p-Akt灰度值分别为(0.035±0.02)和(0.07±0.03),差异有统计学意义(t=4.341、P=0.001);p65灰度值分别为(0.045±0.02)和(0.085±0.04),差异有统计学意义(t=4.000、P=0.003)。结论GBS阳性病例早产可能与阴道分泌物中IL-6、p-STAT3、p-Akt和p65高表达有密切关系。

Objective To investigate the expression of interleukin 6(IL-6)and phosphorylation signal transduction and transcription activation factor 3(p-STAT3)in B group of Streptococcus(GBS)-positive preterm pregnant women and the relationship with preterm birth.Methods From June 2018 to June 2019,a total of 1950 pregnant women were selected from the outpatient antenatal examination to Maternal and Child Health Hospital,Futian District waiting for delivery,among whom,122 cases with GBS positive.Vaginal secretions from 20 cases with GBS positive premature delivery(premature delivery group),33 cases with full-term premature rupture of membranes(premature rupture of membranes),66 cases with full-term vaginal delivery(full-term delivery group),3 cases with abortion(abortion group)and 20 cases with GBS negative(GBS negative control group)were suspended,the concentrations of IL-6 were detected by ELISA method,the expressions of p-STAT3,p-Akt and NF-κB subunit p65 were detected by Western blot.Results The GBS positive rate of pregnant women was 6.25%(122/1950),among whom,abortion,premature delivery,fullterm premature rupture of membranes and full-term delivery accounted for 2.45%(3/122),16.12%(20/122),26.62%(33/122)and 53.66%(66/122),respectively.The expression of IL-6 in GBS positive premature delivery group[(0.065±0.034)pg/ml]was significantly higher than that of full-term delivery group[(0.045±0.021)pg/ml](t=-3.192,P=0.002)and GBS negative control group[(0.043±0.020)pg/ml](t=-2.494,P=0.017),both with significant differences.p-STAT3 expressions in GBS positive premature delivery group(gray value:0.06±0.03)was significantly higher than that of GBS negative control group(gray value:0.04±0.01),with significant difference(t=-2.9812,P=0.005);The gray values of p-Akt and p65 in GBS positive premature delivery group and GBS negative control group[p-Akt:(0.035±0.02)vs.(0.07±0.03),p65:(0.045±0.02)vs.(0.085±0.04)]were with significant differences(t=4.341,P=0.001;t=4.000,P=0.003).Conclusions Preterm delivery in GBS positive pregnant women may be closely related to high expression of IL-6,p-STAT3,p-Akt and p65 in vaginal discharge.