外泌体提取方法及其miRNA在非小细胞肺癌中的应用

Exosome extraction method and application of miRNA in non-small cell lung cancer

作为癌症诊断有前景的生物学标志物,外泌体在癌症的液体活检中受到很大关注。外泌体的生物学价值和临床应用价值凸显了开发有效提取和分离外泌体技术的重要性和必要性。外泌体miRNA越来越被认为是潜在的疾病非侵入性生物学标志物,有越来越多的证据表明外泌体miRNA表达失调与非小细胞肺癌的发生和发展相关。该文对几种不同外泌体分离提取方法及其miRNA在非小细胞肺癌中的作用进行综述。

As a promising biomarker for cancer diagnosis,exosomes have received a lot of attention in liquid biopsy of cancer.The biological value and clinical application value of exosomes highlight the importance and necessity of developing effective techniques for extracting and isolating technology of exosomes.Exosomal miRNAs are increasingly recognized as potential non-invasive biomarkers for disease,and there is increasing evidence that misexpression of exosomal miRNAs is associated with the occurrence and development of non-small cell lung cancer.This review mainly introduces several different methods of exosome isolation and extraction and the role of miRNA in non-small cell lung cancer.