长链非编码RNA结肠癌相关转录物2(CCAT2)在膀胱癌中的表达及作用机制

Expression and mechanism of long non-coding RNA colon cancer-related transcript 2(CCAT2)in bladder cancer

目的探讨长链非编码RNA(lncRNA)结肠癌相关转录物2(CCAT2)在膀胱癌中的表达及作用机制。方法选取膀胱癌组织及癌旁正常组织、膀胱癌细胞(EJ、BIU87、T24、T24-ADM)及膀胱正常上皮细胞(sv-HUC-1),采用qRT-PCR检测CCAT2表达。选取膀胱癌细胞,分别转染siRNA-NC和siRNA-CCAT2,采用qRT-PCR检测细胞中CCAT2表达;采用CCK8法检测细胞增殖活性;流式细胞仪检测细胞凋亡;划痕实验检测细胞迁移;Western blot法检测凋亡相关蛋白表达。结果 CCAT2在膀胱癌组织中的表达明显高于癌旁正常组织(P<0.05);与sv-HUC-1细胞比较,CCAT2在EJ、BIU87、T24、T24-ADM细胞中的表达明显增高(P<0.05),其中以EJ、BIU87细胞最高。与转染siRNA-NC相比较,转染siRNA-CCAT2后细胞中CCAT2表达显著降低(P<0.05)。与转染siRNA-NC相比较,转染siRNA-CCAT2后细胞增殖活性显著降低(P<0.05)。与转染siRNA-NC相比较,转染siRNA-CCAT2后细胞凋亡率显著增高(P<0.05)。与转染siRNA-NC相比较,转染siRNA-CCAT2后细胞迁移能力显著减弱(P<0.05)。与转染siRNA-NC相比较,转染siRNA-CCAT2后细胞Bcl-2蛋白表达显著减少,而Bax蛋白表达显著增高(P<0.05)。结论 CCAT2在膀胱癌组织和细胞中高表达,沉默CCAT2可抑制膀胱癌细胞增殖和迁移,并促进膀胱癌细胞凋亡,其机制可能与调控Bcl/Bax比率有关。

Objective To evaluate the expression and mechanism of long non-coding RNA(lncRNA)colon cancerrelated transcript 2(CCAT2)in bladder cancer.Methods Bladder cancer tissues and paracancer normal tissues,bladder cancer cells(EJ,BIU87,T24,t24-adm)and bladder normal epithelial cells(sv-huc-1)were selected.CCAT2 expression was detected by qRT-PCR.Bladder cancer cells were selected and transfected with siRNA-NC and siRNACCAT2,respectively.The CCAT2 expression was detected by qRT-PCR.The cell proliferation activity was detected by CCK8 method.The apoptosis was detected by flow cytometry.The cell migration was detected by scratch test.The expression of apoptosis-related proteins was detected by Western blot.Results CCAT2 expression in bladder cancer tissues was significantly higher than that in paracancer tissues(P<0.05).Compared with sv-huc-1 cells,CCAT2 was significantly increased in EJ,BIU87,T24 and t24-adm cells(P<0.05),with the highest expression in EJ and BIU87 cells.Compared with transfected siRNA-NC,CCAT2 expression in sirna-ccat2 cells was significantly reduced(P<0.05).Compared with transfected siRNA-NC,the proliferation activity of siRNA-CCAT2 cells was significantly reduced(P<0.05).Compared with transfected siRNA-NC,the apoptosis rate was significantly increased after transfected siRNACCAT2(P<0.05).Compared with transfected siRNA-NC,transfected siRNA-CCAT2 significantly reduced cell migration capacity(P<0.05).Compared with transfected siRNA-NC,Bcl-2 protein expression was significantly reduced and Bax protein expression was significantly increased after transfected siRNA-CCAT2(P<0.05).Conclusion CCAT2 is highly expressed in bladder cancer tissues and cells.Silencing CCAT2 can inhibit the proliferation and migration of bladder cancer cells,and promote the apoptosis of bladder cancer cells.The mechanism may be related to the regulation of Bcl/Bax.