高压脱脂辣椒籽分离蛋白提取工艺优化及其功能特性研究

Study on Extraction Process Optimization and Functional Properties of High-pressure Degreased Capsicum Seed Protein Isolate

为开发利用辣椒籽资源,以辣椒籽为原料,研究高压脱脂辣椒籽分离蛋白的提取工艺及其功能特性。以辣椒籽分离蛋白提取量为考察指标,在单因素实验基础上,研究pH、料液比、提取时间和提取温度对辣椒籽分离蛋白提取量的影响,采用响应面优化法确定最佳提取工艺。结果表明,高压脱脂辣椒籽分离蛋白的最佳提取条件为pH 9.5、料液比1∶39、37℃、38min,提取量达6.27g/100g,等电点(PI)为4,纯度为90.29%。高压脱脂辣椒籽分离蛋白的持水力(3.57±0.12)g/g和持油力(3.15±0.17)g/g较溶剂脱脂辣椒籽分离蛋白分别提高了46.31%和34.04%,说明高压脱脂分离蛋白具有较好的功能特性。

In order to develop and utilize the resources of Capsicumseeds,the extraction technology and functional properties of high-pressure defatted Capsicum seeds protein isolate are studied using Capsicumseeds as the materials.Based on the single factor experiment,the extraction yield of Capsicumseeds protein isolate is taken as the index,the effects of pH values,ratios of solid to liquid,extraction time and extraction temperatures on the extraction yield of Capsicumseeds protein isolate are studied,and the optimal extraction process is determined by response surface methodology.The results show that the optimum extraction conditions are pH 9.5,solid-liquid ratio 1∶39,37℃,38min,the extraction yield is 6.27g/100g,isoelectric point(PI)is 4,and the purity is,and the purity 90.29%.The water holding capacity(3.57±0.12)g/g and oi l holding capacity(3.15±0.17)g/g of higdefatted Capsicum seeds protein isolate Capsicum seeds protein isolate,indicating that hig is 46.31%and 34.04%high erthan that o f solvent degreased h-pressure degreased protein isolate has better functional properties