微囊泡产生及鉴定体系现状分析

Analysis of Microvesicle Generation and Identification System

近年来,随着对微囊泡研究的深入,如何有效获取足量、高纯度的微囊泡成为该领域研究的挑战。微囊泡又称微粒,是细胞激活、损伤或凋亡后从细胞膜脱落的小囊泡,直径为100~1000 nm。微囊泡可通过不同机制与细胞相互作用,发挥与来源细胞相似的生物学功能。与此同时,微囊泡又因其含有天然的细胞膜成分,具有良好的生物传递性,成为仿生药物载体研究的新宠。从原理、应用和关键技术等几个方面综述该领域的研究进展,将目前已有的微囊泡提取及鉴定关键技术做进一步论述,并对微囊泡应用前景进行展望。

In recent years,with microvesicles(MV)studies development,how to effectively obtain a sufficient amount of high purity microvesicles has become a big challenge in this field.Microvesicles also known as microparticles,are small vesicles that fall off from the cell membrane after cell activation,injury or apoptosis.MV with a diameter of about 100~1000 nm can interact with cells through different mechanisms and play a similar biological function as the source cells do.At the same time,MV have become a new favorite in bionic drug carrier research because of their natural membrane components and good biological transmissibility.In this paper,we summarized the principle,application and key technologies,and the existing key technologies for extraction and identification of MV,and also made prospections about MV’s application in the future.