NR3C1表达与前列腺癌生化复发的相关性研究

Correlation analysis between the expression level of NR3C1 and biochemical recurrence of prostate cancer

目的研究NR3C1(核受体亚科3,C组,成员1)又称糖皮质激素受体(GR)表达量对前列腺癌恶性程度的影响及其与前列腺癌生化复发的相关性。方法通过组织芯片免疫组化染色检测的方法检验NR3C1在不同恶性程度前列腺癌组织的表达情况,结合Taylor数据库分析NR3C1表达水平与前列腺癌临床病理特征关系,再采用Kaplan-Meier法分析NR3C1对前列腺癌生化复发生存率的影响,最后用Cox回归分析临床病理特征与生化复发的相关性。结果组织芯片免疫组化结果显示NR3C1在Gleason评分低的前列腺癌组织中表达高于Gleason评分高的前列腺癌组织(P=0.028)。结合Taylor公用数据库分析,NR3C1在前列腺癌组织中的表达低于癌旁组织(P<0.001),NR3C1在Gleason评分低的前列腺癌组织中表达高于Gleason评分高的前列腺癌组织(P=0.005),NR3C1低表达与PSA复发(P=0.028)和转移(P=0.003)相关。Kaplan-Meier结果提示:NR3C1高表达组患者术后的生化复发生存率更高(P=0.043),总体生存率没有明显区别(P=0.872)。单因素分析结果显示:NR3C1(P=0.002),病理分期(P<0.001),Gleason评分(P<0.001),是否转移(P=0.012)是前列腺癌生化复发的影响因素。多因素分析结果显示:高Gleason评分(P=0.017)和转移(P<0.001)均为生化复发危险因素。结论NR3C1影响前列腺癌的发病进程,检验NR3C1的表达情况,能预测前列腺癌患者生化复发的概率,可协助判断前列腺癌预后。

Objective We study the role of NR3C1(nuclear receptor subfamily 3,group C,member 1)in PCa progression,and the correlation between its expression level and the biochemical recurrence of PCa.Methods Immunohistochemistry was used to detect the expression of NR3C1 in PCa tissues of different degrees of malignancy.The associations of NR3C1 expression and clinical pathological features were analyzed using the Taylor dataset.Kaplan-Meier was used to detect the relationship between NR3C1 expression and biochemical recurrence survival rate in PCa.Cox-regressive analysis was used to detect the relationship between clinical pathological features and biochemical recurrence.Results Immunohistochemistry analysis showed the expression of NR3C1 was higher in which its Gleason Score was lower(P=0.028).Base on the Taylor dataset,the expression of NR3C1 was higher in the adjacent benign tissues than that in PCa(P<0.001).The expression of NR3C1 was higher in which its Gleason Score was lower(P=0.005).Furthermore,low NR3C1 expression was associated with PSA failure(P=0.028)and Metastasis(P=0.003).Kaplan-Meier showed the biochemical recurrence-free time of PCa patients in low NR3C1 expression groups reduced(P=0.043).The overall survival time of PCa patients was not correlated to NR3C1 expression levels(P=0.872).Single factor analysis showed the biochemical recurrence is associated with NR3C1 expression(P=0.002),pathological stage(P<0.001),Gleason score(P<0.001),Metastasis status(P=0.012).Multivariate analysis by Cox regression further identified the high Gleason Score(P=0.017)and Metastasis status(P<0.001)were hazards of the biochemical recurrence.Conclusion Our study showed that the expression of NR3C1 critically connected with the process of PCa,which indicated that we can predict the probability of the biochemical recurrence and determine the prognosis of prostate cancer by detecting the expression of NR3C1 in PCa patients.