河源地区机采血小板固定献血者血小板抗原系统基因多态性分析

The polymorphism of human platelet antigens in fixed apheresis platelet donors in Heyuan area

目的探讨河源地区机采血小板固定献血者血小板抗原系统的基因多态性特征,为建立本地区机采血小板供血者库奠定基础。方法采用PCR-SSP方法对100例机采血小板固定献血者进行血小板抗原HPA1~17系统基因分型。结果HPA1~17基因中成多态性分布的等位基因是HPA2a、HPA3a、HPA5a、HPA15a,其频率分别为0.96、0.49、0.99、0.515。HPA-2、HPA-3、HPA-5、HPA-15系统存在aa、ab、bb三种表型。HPA1a、HPA4a、HPA6a-14a、HPA16a-17a基因频率为1,呈单线性分布,未发现b基因。结论河源地区血小板HPA-3系统不配合率最高(0.420),HPA-15系统次之。建立本地区机采血小板供血者库,为患者提供HPA相合的血小板,对减少临床血小板输注无效的发生具有重要意义。

Objective To study the polymorphism of human platelet antigens in fixed apheresis platelet donors in Heyuan area and to lay a foundation for the establishment of platelets donor bank.Methods PCR-SSP method was used to analyze HPA 1~17 genotype in 100 fixed platelet donors.Results The highest numbers of heterozygotes were HPA2a,HPA3a,HPA5a and HPA15a,with frequencies of 0.96,0.49,0.99 and 0.515,respectively.The frequencies of HPA1a,HPA4a,HPA6a-14a and HPA16a-17a genes were 1,which showed a single linear distribution.Conclusion HPA-3 system were the highest mismatch rate(0.420),followed by HPA-15 system.It is great significance to establish a local platelet donor bank and provide HPA compatible platelets for patients.