几种纳米荧光能量受体的比较

Comparison of Several Nanomaterials as Fluorescent Energy Acceptor

本文以荧光素(FAM)为荧光供体,通过与6种不同的纳米材料(还原型氧化石墨烯、氧化石墨烯、纳米Au、纳米Pd、纳米WS2和纳米MoS2)组成能量供-受体对,以DNA为作用媒介,从理论上和实验上比较6种纳米材料对FAM的荧光猝灭效果,希望从中确定最佳的荧光能量受体及其荧光共振能量转移(FRET)相关性质,为荧光传感器提供基础研究数据。研究表明,6种纳米材料中,还原型氧化石墨烯的猝灭效果最好,且还原型氧化石墨烯和纳米Au表现出了长距离FRET的性质。

F rster resonance energy transfer(FRET)-based fluorescent sensors have advantages of high sensitivity,convenience,and so on.Basically,a suitable energy acceptor is very important to FRET,which will efficiently decrease the fluorescence background and improve the sensitivity of sensors.Nowadays,more and more nanomaterials with special optical properties have been found to be very adoptable for fluorescence acceptors,and become the current research focus.In this paper,6 nanomaterials(reduced graphene oxide,graphene oxide,Au nanoparticle,Pd nanoparticle,WS2 nanosheet and MoS2 nanosheet)were used to conduct FRET with FAM as the fluorescence donor,and the fluorescence-quenching performance of these nanomaterials was compared,in purpose of finding out the best nano-quencher for fluorescent sensors.DNA was used to control the distance between fluorescence donor and acceptor,which was essential to FRET.The results showed that,reduced graphene oxide performed the best quenching to FAM,and the quenching efficiency was 95%under a concentration of 3μg/mL.Additionally,long-range FRET was found to be one of the excellent properties of reduced graphene oxide and Au nanoparticle,which could display FRET quenching to FAM in 30 nm donor-acceptor distance.