氢气影响大鼠神经兴奋传导的研究

Study on Effect of Hydrogen on Excitatory Conduction in Rats

氢气是一种具有重要生物学功能的气体分子,可以用于神经退行性疾病、抑郁、睡眠障碍和毒瘾戒断症状等的治疗和改善,普遍认为和氢气的选择性抗氧化有关,但氢气对神经功能的调控机制尚不清楚。为了探究氢气对神经功能的调控机制,通过脑片膜片钳技术分别检测了氢瞬时作用大鼠大脑切片皮层神经细胞和饮用富氢水(8周)大鼠大脑切片皮层神经细胞的动作电位变化,以判断氢的干预是否能够影响神经兴奋的传导;利用液相色谱质谱联用仪(liquid chromatograph-mass spectrometer,LCMS)检测饮用富氢水(8周)大鼠大脑切片皮层神经递质的含量变化,以进一步探究氢气影响神经兴奋传导的具体机制。结果表明,氢气处理组与对照组相比大鼠皮层神经细胞的阈值电压、动作电位间隔和输入抗阻具有显著性差异(P<0.05),氢气处理组静息膜电位升高,神经细胞爆发动作电位阈值升高,表明氢气可能对神经细胞膜离子通道的开放和关闭有影响,氢处理能够使皮层神经细胞兴奋性明显降低。大鼠在连续饮用富氢水8周后大脑皮层同样显现出兴奋性降低趋势,经LCMS测定,发现神经递质的含量没有明显变化。研究提示,氢气可能是通过改变细胞内外电荷差异变化或者直接影响神经细胞表面钠、钾等离子通道的打开或关闭,从而实现对神经细胞兴奋性的调节。

Hydrogen is a gas molecule with important biological functions,which can be used for the treatment and improvement of neurodegenerative diseases,depression,sleep disorders and drug addiction withdrawal symptoms.It is generally believed that it is related to the selective oxidation resistance of hydrogen,but the regulatory mechanism of hydrogen on neural function has not been explained clearly.In order to explore the regulation mechanism of hydrogen on neural function,the action potential changes of cortical neurons in cerebral slices of rats exposed to hydrogen transiently and cortical neurons in cerebral slices of rats that drinking hydrogen-rich water for 8 weeks were detected by patch clamp technique to determine whether hydrogen intervention can affect the conduction of nerve excitation.Liquid chromatography-mass spectrometry(LCMS)was used to detect the changes of neurotransmitters in cerebral slices of rats drinking hydrogen-rich water(eight weeks)in order to further explore the specific mechanism of hydrogen affecting nerve excitation conduction.The results showed that compared with the control group,the threshold voltage,action potential interval and input impedance of rat cortical neurons in the hydrogen treatment group had significant difference(P<0.05).The resting membrane potential in the hydrogen treatment group increased,and the threshold of nerve cell burst action potential increased,which indicated that hydrogen might affect the opening and closing of ion channels in nerve cell membrane,and hydrogen treatment could significantly reduce the excitability of cortical neurons.After drinking hydrogen-rich water continuously for eight weeks,the cerebral cortex of rats also showed a decrease in excitability,and LCMS showed no significant change in neurotransmitter content.Research suggested that hydrogen may regulate the excitability of nerve cells by changing the change of charge difference inside and outside cells or directly affecting the opening or closing of sodium and potassium plasma channels on the surface of nerve cells.