氯化高铁血红素诱导K562细胞红系分化对其细胞周期的影响

The Effect of Hemin-induced Erythroid Differentiation in K562 Cells on Cell Cycle

细胞周期的测量是细胞增殖动力学的研究基础。通过添加30μmol·L-1氯化高铁血红素(Hemin)诱导人慢性髓系白血病K562细胞红系分化,利用5-溴脱氧尿嘧啶核苷(BrdU)与7-AAD双染的方法检测Hemin诱导的K562红系分化细胞对细胞周期各期比例的影响,未诱导的K562细胞周期各期比例作为对照,检测发现Hemin诱导的K562红系分化细胞对其细胞周期相对值无明显影响。应用BrdU间隔染色结合流式细胞术的方法,通过分析BrdU间隔染色后BrdU阳性细胞群的动态变化规律,从而推算出K562红系分化细胞的倍增时间及细胞周期各期时长。根据测量结果发现,未诱导的K562细胞总倍增时间约为20 h,与通过生长曲线公式法计算倍增时间的结果相符,Hemin诱导的K562细胞的细胞周期倍增时长约为23 h。Hemin诱导的K562红系分化细胞较未诱导的K562细胞倍增时间与各期时长无明显差异。因此,Hemin诱导K562细胞红系分化对其细胞周期绝对值及相对值均无明显影响。

Cell proliferation kinetics based on the measurement of cell cycle length.In this paper,30μmol·L-1 Hemin induced erythroid differentiation of human chronic myeloid leukemia K562 cells.Hemin-induced K562 erythroid differentiated cells were detected the effect on the percentage of cell cycle phases by using 5-bromodeoxyuridine(BrdU)and 7-AAD double staining.The percentage of each phase of K562 cells were used as control.It was found that Hemin-induced K562 erythroid differentiated cells had no significant effect on the relative value of cell cycle.Interval staining combined with flow cytometry were used to analyze the dynamic change of BrdU positive cell population so that the doubling time and cell cycle duration of erythroid differentiated cells of K562 cells were calculated.According to the measurement results,the total doubling time of K562 cells was about 20 hours,which was consistent with the calculation of the doubling time by growth curve methods.The cell cycle doubling time of K562 cells induced by Hemin was about 23 hours.The doubling time of K562 cells induced by Hemin was not significantly different from K562 cells.Therefore,Hemin-induced erythroid differentiation of K562 cells had no significant effect on the absolute value and relative value of cell cycle.