慢性牙周炎患者外周血单核细胞Notch1 mRNA的表达及与疾病严重程度的相关性

Level of Notch1 mRNA in peripheral blood mononuclear cells of patients with chronic periodontitis and its correlation with disease severity

目的探讨慢性牙周炎患者外周血单核细胞Notch1 mRNA的表达及与疾病严重程度的相关性。方法选取2017年1月—2018年12月丽水市人民医院口腔科收治的慢性牙周炎患者150例作为观察组,另取同期该院150例健康体检者作为对照组。观察组根据病情严重程度分为轻度组54例,中度组51例,重度组45例。采用实时荧光定量聚合酶连反应检测外周血Notch1 mRNA表达,流式细胞仪测定外周血辅助性T细胞17(Th17)水平,双抗体夹心法酶联免疫吸附试验测定外周血白细胞介素-17(IL-17)水平。结果观察组外周血Notch1 mRNA表达,Th17细胞、IL-17水平均高于对照组(P<0.05)。不同严重程度慢性牙周炎患者外周血Notch1 mRNA表达,Th17细胞、IL-17水平比较差异有统计学意义(P<0.05),中度组和重度组高于轻度组(P<0.05),重度组高于中度组(P<0.05)。慢性牙周炎患者治疗后外周血Notch1 mRNA表达,Th17细胞、IL-17水平低于治疗前(P<0.05)。慢性牙周炎患者治疗前外周血Notch1 mRNA表达与Th17细胞、IL-17水平呈正相关(r=0.548和0.572,P<0.05)。结论Notch1 mRNA可能通过促进Th17细胞分化参与慢性牙周炎的发病过程,Notch1 mRNA表达水平可在一定程度上反映慢性牙周炎的病情严重程度。

Objective To investigate the level of Notch1 mRNA in peripheral blood mononuclear cells of patients with chronic periodontitis and its relationship with disease severity.Methods 150 patients with chronic periodontitis treated in the Department of Stomatology of Graduate School of Zhejiang University of Traditional Chinese Medicine and Lishui People’s Hospital from January 2017 to December 2018 were selected as the observation group,and 150 healthy people who underwent physical examination during the same period were selected as the control group.According to the severity of the disease,the observation group patients were divided into mild group(54 cases),moderate group(51 cases),and severe group(45 cases).Real-time fluorescence quantitative polymerase chain reaction was used to determine Notch1 mRNA levels in peripheral blood.Flow cytometry was used to determine T helper 17(Th17)cells in peripheral blood.Double-antibody sandwich ELISA was used to measure interleukin-17(IL-17)levels in peripheral blood.Results The levels of Notch1 mRNA,Th17 cells and IL-17 in the observation group were higher than those in the control group(P<0.05).There were significant differences in the levels of Notch1 mRNA,Th17 cells and IL-17 in peripheral blood of patients with different severity of chronic periodontitis(P<0.05),where those in the moderate group and severe group were higher than those in the mild group(P<0.05)and those in the severe group were higher than those in the moderate group(P<0.05).The levels of Notch1 mRNA,Th17 cells and IL-17 in peripheral blood of patients with chronic periodontitis after treatment were lower than those before treatment(P<0.05).The levels of Notch1 mRNA in peripheral blood of patients with chronic periodontitis before treatment were positively correlated with the levels of Th17 cells(r=0.548)and IL-17(r=0.572)(P<0.05).Conclusions Notch1 may participate in the pathogenesis of chronic periodontitis by promoting the differentiation of Th17 cells.The level of Notch1 mRNA can reflect the severity of chronic periodontitis to a certain extent.