TACC3 mRNA在乳腺癌中的表达及其基因富集分析

Expression of TACC3 mRNA in breast cancer and its gene set enrichment analysis

目的分析TACC3 mRNA在乳腺癌中的表达水平及其与预后的关系;探讨TACC3 mRNA表达与乳腺癌患者临床资料的关系;运用GSEA基因富集分析方法预测TACC3所调控可能影响乳腺癌发展的基因集。方法登录GEO数据库下载并收集与乳腺癌相关的基因表达谱GSE42568及对应的临床信息,分析在GSE42568数据中104例乳腺癌和17例正常乳腺组织样本TACC3 mRNA表达的差异、临床资料及预后;利用GSEA基因富集分析的方法预测受TACC3调控的相关基因。结果TACC3 mRNA在乳腺癌癌组织中呈高表达,差异具有统计学意义(P=0.001);TACC3 mRNA在不同年龄、肿瘤分级、淋巴结转移、雌激素受体、T分期组间的表达均无差异(P>0.05);生存分析中,高表达TACC3 mRNA的患者总体生存期低于低表达患者(P=0.009);TACC3 mRNA高表达样本富集了细胞周期、DNA复制、RNA降解、原发性免疫缺陷、T细胞受体信号通路、剪接体等相关基因集。结论TACC3 mRNA在乳腺癌中高表达,TACC3与乳腺癌患者的预后有关,有可能成为乳腺癌的新诊疗靶点。

Objective To analyze the expression level of TACC3 mRNA in breast cancer and its relationship with prognosis,and to investigate the relationship between TACC3 mRNA expression and clinical data of breast cancer patients.Gene set enrichment analysis(GSEA)was used to predict the gene set regulated by TACC3 that may affect the development of breast cancer.Methods The expression profile GSE42568 and corresponding clinical information related to breast cancer were collected from GEO database.TACC3 mRNA expression differences,clinical data and prognosis of 104 breast cancer and 17 normal breast tissue samples from GSE42568 data were analyzed.Related genes regulated by TACC3 were predicted through GSEA.Results TACC3 mRNA was highly expressed in breast cancer tissues,the difference was statistically significant(P=0.001).There was no difference in TACC3 mRNA expression among groups with different age,tumor grade,lymph node metastasis,estrogen receptor and T stage(P>0.05).In the survival analysis,the overall survival time of the patients with high expression of TACC3 mRNA was shorter than that of the patients with low expression of TACC3 mRNA(P=0.009).The high expression samples of TACC3 mRNA were enriched in cell cycle,DNA replication,RNA degradation,primary immune deficiency,T cell receptor signaling pathway,spliceosome and other related gene sets.Conclusion TACC3 mRNA is highly expressed in breast cancer.TACC3 is related to the prognosis of breast cancer patients and may become a new target for the diagnosis and treatment of breast cancer.