雪旺细胞对种植体周围组织修复的实验研究

An experimental study of Schwann cells on tissue repair around implants

目的:探索雪旺细胞移植治疗是否对种植体周围牙龈组织具有修复作用。方法:体外培养比格犬坐骨神经雪旺氏细胞,免疫荧光染色鉴定。6只比格犬双侧下颌后牙拔出后2月,自体坐骨神经雪旺细胞(实验组)或生理盐水(对照组)分别与种植体共移植。ELISA试剂盒观察龈沟液中炎症因子(TNF-α、IL-1β和IL-6)和神经营养因子(BDNF、NGF)表达,免疫组织化学染色观察牙龈组织NF-200表达。应用SPSS17.0进行数据统计分析。结果:体外培养的细胞为S-100阳性。实验组神经元轴突长于对照组(P<0.05)。龈沟液中TNF-α、IL-1β和IL-6的表达低于对照组(P<0.05),BDNF和NGF的表达高于对照组(P<0.05),NF-200(+)神经纤维更多。结论:雪旺细胞可以促进种植体周围组织修复。

Objective:To explore whether Schwann cell transplantation can promote the repair of the tissue around implants.Meth-ods:Schwann cells were in vitro cultured and indentified by immunocytofluorescence.Bilateral mandibular posterior teeth were extrac-ted in 6 beagle dogs,implantation was performed with Schwann cells(test group,n=6)or normal saline(control group,n=6).The.expression of inflammatory factors(TNF-α,IL-1β,IL-6)and nerve growth factors(BDNF,NGF)in gingival crevicular fluid(GCF)were detected by ELISA kit,the NF-200(+)axons was detected by immunohistochemistry.SPSS 17.0 was used for statistical analy-sis.Results:The cultured cells from beagle dogs were S-100 positive.One month after implantation the neurite length of test group waslonger(P<0.05),the expression of TNF-α,IL-1βand IL-6 in GCF were lower(P<0.05),the expression of BDNF and NGF werehigher(P<0.05),the NF-200(+)axons were more(P<0.05)than those of the control group.Conclusion:Schwann cells play animportant role in promoting tissue repair around implants after implantation.