病毒灭活血样对输血相容性检测的影响

Effect of Virus Inactivated Blood Sample on Blood Transfusion Compatibility Test

目的探讨新冠肺炎流行形势下,血液样本经56℃30 min病毒灭活处理后对部分血型抗原性及抗体效价的影响。方法选取20例不同血型样本和6例含不规则抗体样本,在样本加热灭活处理前后,采用微柱凝集试验分别检测血液样本部分血型抗原性和抗体效价。根据反应凝集强度判断ABO血型抗原性,抗体效价以凝集强度1+为判定标准,比较热灭活前后样本红细胞ABO抗原性和血浆中抗体效价的变化。结果56℃30 min灭活处理的血液样本溶血严重,对ABO血型抗原检测无影响,不规则抗体效价降低1~2个滴度,对效价≤4的抗体会漏检,对效价>4的抗体检出无影响。结论血液样本热灭活,对ABO血型系统以外抗体效价有一定影响,但能有效降低操作者的职业暴露风险。

Objective The effect of novel coronavirus pneumonia on the antigenicity and antibody titer of some blood groups after 56℃30 min of virus inactivation was detected.Methods 20 samples with different blood types and 6 samples with different irregular antibodies were selected.Before and after the samples were heated and inactivated,the antigenicity and antibody titer of the samples were detected by microcolumn gel method.The antibody titer was determined by the agglutination intensity of 1+,and the changes of antigenicity and antibody titer of the samples before and after thermal inactivation were compared.Results The hemolysis of blood samples inactivated at 56℃for 30 minutes was serious,which had no effect on the detection of ABO blood group antigen.The titer of irregular antibody decreased by 1~2 titers,the antibody with titer≤4 would be missed,and the antibody with titer>4 would not be affected.Conclusion The heat inactivation of blood samples has certain influence on the antibody titer outside the ABO blood group system,but it can effectively reduce the risk of occupational exposure of operators.