摘要
目的:研究布洛芬对慢性癫痫大鼠海马区环氧化酶2(COX-2)和神经元兴奋性的影响。方法:选用雄性健康SD大鼠48只,随机分为3组,分别为对照组(control)、癫痫组(epilepsy)和治疗组(ibuprofen)。运用戊四氮(PTZ)诱导建立慢性癫痫大鼠模型并用布洛芬干预,采用行为学观察检测大鼠癫痫发作水平,运用免疫组织化学染色和Western Blot技术检测大鼠海马区COX-2的表达情况,运用全细胞膜片钳技术收集并分析大鼠海马区动作电位的变化情况。结果:与对照组相比,癫痫组大鼠癫痫发作明显,海马区COX-2表达升高,海马神经元兴奋性升高(P<0.05);治疗组大鼠癫痫发作等级降低,海马区COX-2表达明显降低,海马神经元兴奋性降低(P<0.05)。结论:布洛芬通过下调COX-2降低癫痫大鼠海马神经元的兴奋性,降低癫痫发作强度,具有一定的保护作用。
Objective: To investigate the effects of ibuprofen on cyclooxygenase 2(COX-2) and excitability of hippocampal neurons in chronic epileptic rats.Methods: Forty-eight healthy male Sprague-Dawley rats were randomly divided into three groups: control group(control),epilepsy group(epilepsy),and ibuprofen treatment group(ibuprofen).Rat model of chronic epilepsy was prepared by intraperitoneally injection of pentylenetetrazol(PTZ).The rats of epilepsy group were treated with ibuprofen.The seizure score of rats was measured by behavioral test.The expression of COX-2 in the hippocampus of rats was detected by immunohistochemical staining and Western Blot.Whole-cell patch-clamp was used to collect and analyze the changes of the action potential in hippocampal neurons.Results:Compared with control group,the seizure score,expression of COX-2 and excitability of hippocampal neurons were significantly increased in the epilepsy group(P < 0.05);The seizure score,expression of COX-2 and excitability of hippocampal neurons in the treatment group were lower than those in the epilepsy group(P < 0.05).Conclusion: Ibuprofen can affect seizures by down-regulating the expression of COX-2 and reducing the excitability of hippocampal neurons in epileptic rats.
作者
刘睿
郭翀
彭江涛
胡忠波
郭科
张鑫帆
吴淑华
李建民
Liu Rui;Guo Chong;Peng Jiangtao;Hu Zhongbo;Guo Ke;Zhang Xinfan;Wu Shuhua;Li Jianmin(Department of Neurosurgery,Binzhou Medical University Hospital,Binzhou 256603,China;Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,China)
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2020年第4期383-388,共6页
Chinese Journal of Neuroanatomy
基金
山东省医药卫生科技发展计划项目(2017WS553)
滨州医学院科技计划项目(BY2015KJ13)。
关键词
癫痫
布洛芬
环氧化酶2
膜片钳
动作电位
大鼠
epilepsy
ibuprofen
cyclooxygenase 2(COX-2)
whole-cell patch-clamp
action potential
rat