鞣酸蛋白酵母散对幼年大鼠腹泻的治疗作用及其机制研究

Effects of albumin tannate and barm powder on diarrhea and its diarrhea treatment-related mechanism

目的研究鞣酸蛋白酵母散的治疗作用及其与保护肠黏膜、促进其修复的关系。方法将48只21 d SD大鼠随机分为6组:对照组、模型组、蒙脱石散(阳性药,0.8 g/kg)组和鞣酸蛋白酵母散低、中、高剂量(0.1、0.3、0.9 g/kg)组。除对照组外,所有动物用4 g/kg番泻叶水煎剂连续ig 2周后,ig给药,对照组和模型组给予等体积的蒸馏水,每天2次,给药5 d。记录各组大鼠稀便率和腹泻指数。经治疗后,取各组大鼠空肠做苏木精-伊红染色和免疫组化,分别观察肠黏膜组织结构的改变和肠黏膜增殖细胞核抗原(PCNA)表达的变化。同时,刮取大鼠空肠黏膜分别测定DNA、总蛋白和IL-6的表达水平。结果与模型组比较,经高剂量的鞣酸蛋白酵母散治疗后,大鼠稀便率和腹泻指数显著降低(P<0.001),空肠绒毛结构基本恢复正常,空肠黏膜DNA含量显著增加(P<0.05),PCNA表达显著增加(P<0.001),IL-6表达减少。中剂量的鞣酸蛋白酵母散可改善大鼠腹泻,但对其他指标没有显著影响。而低剂量的鞣酸蛋白酵母散则无作用。结论鞣酸蛋白酵母散对幼年大鼠腹泻有治疗作用,并可促进肠黏膜修复。

Objective To explore the effects of Albumin Tannate and Barm Powder on diarrhea and repairing injury of intestinal mucosa.Methods Forty-eight 21-day-old rats were randomly divided into six groups:control group,diarrhea modeling group,montmorillonite powder(0.8g/kg)group,Albumin Tannate and Barm Powder low,middle,and high does(0.1,0.3,and 0.9 g/kg)group.Except for control group,all rats were given with Senna leaf water decoction(4 g/kg)for two weeks,which was followed by treatment with corresponding drug or distilled water for 5 days.After measuring the diarrhea index,we take the jejunum for H&E stain and immunohistochemistry to evaluate tissue structure or the expression of PCNA.Besides these,scrape the intestinal mucosa to measure the level of DNA,protein and interleukin 6.Results Compared with model group,after the treatment of high dose of Albumin Tannate and Barm Powder,the defecation rate and diarrhea index of rats were significantly reduced(P<0.001),the structure of jejunum villi was basically restored to normal,the DNA content of jejunum mucosa was significantly increased(P<0.05),the expression of PCNA was significantly increased(P<0.001),and the expression of IL-6 was decreased.However,Albumin Tannate and Barm Powder of midle does could only relieve diarrhea symptom and Albumin Tannate and Barm Powder of low does had no effect on diarrhea.Conclusion Albumin Tannate and Barm Powder has good effect on diarrhea and promote the repair of intestinal mucosa.