基于网络药理学探讨蒙药苏龙嘎-4颗粒抗腹泻的作用机制

Study on mechanism of anti-diarrhea of mongolian medicine Sulongga-4 Granules based on network pharmacology

目的运用网络药理学探讨苏龙嘎-4颗粒的多成分、多靶点、多途径的抗腹泻作用机制。方法通过TCMSP平台、BATMAN-TCM数据库及文献检索收集苏龙嘎-4颗粒中4味中药的全部化学成分和作用靶点,同时利用GeneCards数据库和OMIM数据库获取腹泻的已知治疗靶点。构建韦恩图,得到苏龙嘎-4颗粒抗腹泻的作用靶点。采用Cytoscape 3.7.1软件,将活性成分与潜在靶点构建“药物-成分-靶点”网络;通过String 10.0数据库建立PPI网络,并获得核心靶点。采用R软件对交集基因进行GO功能分析和KEGG通路分析。结果根据数据库检索,结合所制定的筛选标准,得到苏龙嘎-4颗粒34个活性成分和110个靶点,其中74个靶点为苏龙嘎-4颗粒抗腹泻的潜在靶点。GO生物学过程分析得出87个条目,KEGG通路分析得出117条通路,主要涉及PI3K-Akt信号通路、TNF信号通路、p53信号通路、凋亡通路等。推测苏龙嘎-4颗粒可能通过抗炎、影响细胞凋亡、修复肠粘膜损伤而起到抗腹泻的作用。结论苏龙嘎-4颗粒的活性成分主要通过CASP-3、EGFR、IL-6、VEGFA、MAPK8等靶点抑制炎症因子的产生阻断炎症反应、影响细胞凋亡的同时对肠粘膜损伤的进行修复,最终发挥抗腹泻的作用。

Objective Sulongga-4 Granules is a classic ethnic medicine formula,which is clinically used in the treatment of infantile diarrhea.In this study,the potential mechanism of multi-component,multi-target and multi-pathway treatment of diarrhea was explored based on network pharmacology.Methods Through TCMSP platform,Batman-TCM database and literature retrieval,all the chemical components and targets of four Traditional Chinese medicines in Sulonga-4 Granules were collected.and the known therapeutic targets of diarrhea were obtained by GeneCards database and OMIM database.The Venn diagram was constructed to obtain the therapeutic target of Sulongga-4 Granules in treating diarrhea.Cytoscape 3.7.1 software was used to construct a"drugcomponent-target"network between active ingredients and potential targets.PPI network was established through String 10.0 database and core target proteins were obtained.R software was used to analyze the GO function and KEGG pathway of intersection genes.Results According to the database search and the established screening criteria,34 active components and 110 targets of Sulongga-4 Granules were obtained,among which 74 were potential targets for anti-diarrhea of Sulongga-4 Granules.GO biological process analysis revealed 87 items,and KEGG pathway analysis revealed 117 pathways,mainly involving PI3k-Akt signaling pathway,TNF signaling pathway,p53 signaling,pathway apoptosis pathway,etc.It is speculated that Sulongga-4 Granules may play an anti-diarrhea effect by anti-inflammatory,affecting apoptosis and repairing intestinal mucosal damage.Conclusion The active components of Sulongga-4 Granules mainly inhibit the production of inflammatory factors,block inflammatory response,promote apoptosis and repair intestinal mucosal damage through CASP-3,EGFR,IL-6,VEGFA,MAPK8 and other targets,and finally play an anti-diarrhea role.