Search-and-replace editing of genetic information

Genome editing based on CRISPR/Cas9 system enables unprecedented breakthroughs in genomic research and the engineering of variants associated to clinical diseases[1,2].The CRISPR/Cas9 system induces double strand breaks(DSBs),which are primarily repaired by non-homologous end joining(NHEJ)and low-efficiency homologydirect repair(HDR)pathways.However.